Chlor_00040 Chlor_00040   Chlor_00060 Chlor_00060

Chlor_00050 : CDS information

close this sectionLocation

Organism
StrainA82846UV37B
Entry nameChloroeremomycin
Contig
Start / Stop / Direction25,415 / 30,997 / + [in whole cluster]
25,415 / 30,997 / + [in contig]
Location25415..30997 [in whole cluster]
25415..30997 [in contig]
TypeCDS
Length5,583 bp (1,860 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)PCZA363.5
GenecepC
ORF5
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • similar to peptide synthetase
Reference
ACC
PmId
[9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998)
[17506888] Phylogenetic analysis of condensation domains in NRPS sheds light on their functional evolution. (BMC Evol Biol. , 2007)
comment
[PMID: 9545426](1998)
chloroeremomycin生合成 gene clusterの報告。

ORF5/CepC(1860aa): Peptide synthetase

NRPSに関して配列解析のみ。
A domainの配列から基質を予測。

7: S-DHPG(3,5-dihydroxyphenylglycine)

---
[PMID: 17506888](2007)
NRPS C domain subtypesの系統発生関係の再構築をしている報告。

グリコペプチド系抗生物質のNRPSに含まれるC domain様区域(X*)は、2つのL-AAsの間のペプチド結合を触媒するC domainのsubtypeに関係し、祖先では追加のmoduleがあったと暗示される。

Figure 4の系統樹にこのentryも含まれる。
Related Reference
ACC
Q939Y9
NITE
Balhi_00090
PmId
[11932455] Nonribosomal biosynthesis of vancomycin-type antibiotics: a heptapeptide backbone and eight peptide synthetase modules. (Microbiology. , 2002)
[15651041] The biosynthesis of vancomycin-type glycopeptide antibiotics--a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. (Chembiochem. , 2005)
[17506888] Phylogenetic analysis of condensation domains in NRPS sheds light on their functional evolution. (BMC Evol Biol. , 2007)
comment
BLAST id88%
Amycolatopsis balhimycina_bpsC
Peptide synthetase

---
[PMID: 11932455](2002)
balhimycin生合成gene clusterにおけるbpsA, bpsB, bpsC, bpsD, orf1の同定。

BpsCのドメイン構造は、
module 7: C-A-T-C-TE
adenylation(A) domainのselectivity-conferring codeを確認している。

bpsC in-frame deletion mutantは抗菌活性のある化合物を産生しなかった。
module 7 は L-3,5-dihydroxyphenylglycine(Dpg)を活性化することが、異種性発現して得たA domainのATP/PP(i) exchange assaysによって測定された。

thiolation(T) domainとthioesterase(TE) domainの間にあるC domainに似た配列に割り当てられるペプチド骨格構築での機能はない。

---
[PMID: 15651041](2005)
dpgA mutantではbicyclic hexapeptide、bpsC mutantではmonocyclic hexapeptideの蓄積があることから、OxyBによるCD ring形成はBpsB_module 6からBpsC_module 7へのhexapeptideの移動中かその少し前に起こるはずだと述べている。bpsC mutantでbicyclic hexapeptideが検出されないのは、BpsCでのdeletionのせいでOxyAとNRPS複合体の間の相互作用に必須な部位がないからであると説明されうる。

また、dpgA mutantにおいて、7つめのAAとしてDpgの代わりにL-4-hydroxyphenylglycine(Hpg)を含むtricyclic heptapeptideが得られたことから、BpsCとoxygenasesはいくらかの基質寛容性を示す。

---
[PMID: 17506888](2007)
Figure 4の系統樹にこのentryも含まれる。
ACC
Q70AZ6
NITE
Teicp2_00210
PmId
[25686610] X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. (Nature. , 2015)
[26549530] Sequential In Vitro Cyclization by Cytochrome P450 Enzymes of Glycopeptide Antibiotic Precursors Bearing the X-Domain from Nonribosomal Peptide Biosynthesis. (Angew Chem Int Ed Engl. , 2015)
[27213615] Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. (J Am Chem Soc. , 2016)
comment
BLAST id79%
Actinoplanes teichomyceticus ATCC 31121株_tcp ORF12
Non-ribosomal peptide synthetase

---
[PMID: 25686610](2015)
SUPPLEMENTARY INFORMATION内のリストにこのUniProt entryの記載があるが、同グループの他論文(PMID: 25358800, 25586301)ではDSM43866株を使用してATCC 31121株由来のgene nameを採用している混乱があるため、同様の可能性あり。

X-domainの結晶構造解析や相互作用の実験から、X-domainがcytochrome P450(oxy genesでコードされる)による架橋形成に関与することがわかった。

---
[PMID: 26549530](2015) abstract + supporting information
PMID: 25686610の続報。同様にsupporting informationでこのUniProt entryの記載あり。その他タンパクもATCC 31121株のentriesを採用している。

close this sectionPKS/NRPS Module

A7 L-3,5-dihydroxyphenylglycine(Dpg)
C15..310
A493..889
PCP967..1038
X1052..1351
TE1624..1841

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [PCZA363.5]
MTVDDTRAKPRSSVEDVWPLSPLQQGMLYHTALDNDGPDTYTVQTVYGIDGPLDAGLLRA
SWQALVDRHAALRACFRYVSGAQMVQVIARDAEIPWNETDLSGLPEDIADSEVDRLAAQE
VAERLRIEVAPLMKLHLIRLGPESHRLVHTLHHVLVDGWSMPIIHRELAAIYAAGGDASG
LPPTVSYRDYLAWLGRQDKDVAIAAWRKELAGLETPTTVAPADPTRVPDIDTVVIELSTQ
LTDDLYRLARGHDLTLNTVVQGAWAVVLSQLAGRNDVVFGATASGRPADLPGVEAMVGQL
LTTLPVRVRLDGERRAVDLFAQLQRDQSALMAHQHLGLQDVQAVVGRGAVFDTLVIYENF
PRKGLGRAPDGGLTLLPVKRGRNSSHYPFTLVTGPGEQMPLILDYDRGLFDPAAAESVVG
ALARVLERLVAEPDILVGRLTLVSEAERALVVDEFNATAGPVPGESVVELFAHRVAEAPD
AVAITDAGGANLTYAEVDRASNRLAGYLAGRGVGRGDRVGVAMDRSPELLIAFLAIWKAG
AAYVPVDVEYPAERIAFILNDSGVSTVLCTQDTSGVVPENAIVLDAPDTRASIEDCADTA
PEIRLYAGDLAYVMYTSGSTGLPKGVAIPHGAVAGLAGDSGWQIGPDDGVLMHATHVFDP
SLYAMWVPLVAGGRVLLTEPGVLDAAGVRQAVERGATAVHLTAGSFRALAETSPECFAGL
VEIGTGGDVVPAQSVANLRRAQPGLRVRNTYGPTETTLCATWLPIEPGDVIDRELPIGHP
MTNRKIYILDAFLRPVPPGVAGELYIAGTGLARGYLDSPGLTADRFVACPFLAGERMYRT
GDVARWTRDGEVVFLGRADDQVKIRGYRVELGEVEAVLAAQPGVVEAVVMAREDQPGEKR
LVGYFVSDGSDAGPAEIRRQMAQVLPDYMVPIAVVALPGLPVTPNGKVDRRALPAPDLAG
RSSEKAPENETEKVLCALFGEILSVDQVGVDDTFNDLGGSSALAMRLIARIREELGADLP
IRQLFSSPTPAGLARALAAKSRPALEAAQRPDRVPLTARQLRAWLLARPGEDTADQHVSI
TLRLGGKLNVPALAAAIGDVAARHEILRTTFPGDAQSVHQHVHDALAIELTPVPATEEDL
PGLLAERRESVFDLTQEVPWRCDLFQLSEKDHVLQVKIHRILADDDSMDVFFRDLAAAYG
ARREGRVPERAPLALQFADFAIWEHRLLEDEQELDSLINEQIVFWRDNLAGIDGETVLPF
DRPRSAVPSRRAGTVALRLDADPHARLTAVAEPVGADTLQMVHAALAMLLAKFGAGHDVV
IGTTLPRDEELFDLEPMIGPFTRPLALRTDVSGDPTFLEVVTRVQEAAQAAGEHLDVPFE
RIVELLDLPASLSRHPVFQVGLQVDEEDIDGWDAAELPALRTSVEPGGTEAMELDLAVKL
TERFDDDDNSGGIEGALHYATDLFDEATAESVARRLVRVLEQVAEDPQRRISDLDIFLDD
FERGRPAIAPARWSGAVPAVVADLAQDGPLGALLLDEQLRPVAPGAVGDLYVTGPAVDAG
TAGLPTMPCPFGTPGHQMMHTGLLARKSHTKALVVVGERRRSSASVKTGDFEILLPLRVG
GDRPPLFCVHASGGLSWNYEPLLRYLPPNQPVYGVQARGLARTEPLPGSVDEMAADYLEQ
IRAVQPTGPYHLLGWSLGGRIAQAMAKLLEAEGEQVGLLALLDAYPVYMGRKTTGAASEE
EALERRNQQDLELAGQLVKGAAARSRLEAVMRNLWDVGPRHVSTPFAGDILLFVATVDRP
GHLPVPVAKDSWKDFTSGAIEAHEIASNHYDMVQPAALGQIGAIVAEKLRSRPDAERTQR
selected fasta
>non-ribosomal peptide synthetase [PCZA363.5]
GTGACCGTTGACGACACTCGCGCGAAGCCGCGCTCCAGTGTGGAAGACGTCTGGCCGCTT
TCCCCGCTGCAGCAAGGAATGCTCTACCACACCGCCCTCGACAACGACGGGCCGGACACG
TACACCGTGCAGACCGTCTACGGCATCGACGGCCCGCTGGACGCCGGGCTGCTGAGGGCG
TCGTGGCAGGCCCTCGTGGACCGGCATGCCGCGCTGCGGGCCTGTTTCCGCTATGTCAGC
GGCGCGCAGATGGTGCAGGTCATCGCGCGGGACGCCGAGATTCCGTGGAACGAGACGGAT
CTTTCCGGGCTGCCTGAGGACATCGCCGACAGCGAGGTGGACCGGCTGGCGGCGCAAGAG
GTGGCCGAGCGGCTGCGCATCGAGGTGGCGCCGCTGATGAAACTGCACCTGATCCGGCTC
GGCCCGGAGAGCCACCGGCTCGTGCACACGCTGCATCACGTGCTGGTCGACGGCTGGTCG
ATGCCGATCATCCACCGCGAACTCGCCGCCATCTACGCGGCGGGCGGTGACGCGTCCGGG
CTCCCGCCCACGGTGTCCTATCGGGACTATCTCGCGTGGCTTGGCCGGCAGGACAAGGAC
GTTGCCATCGCGGCATGGCGCAAGGAGCTGGCCGGGCTGGAGACGCCGACCACGGTCGCC
CCGGCCGATCCGACCCGCGTGCCGGACATCGACACAGTCGTGATCGAGTTGTCCACACAG
CTGACGGACGATCTGTACCGGCTAGCGCGCGGCCACGACCTCACCCTGAACACGGTCGTG
CAGGGCGCTTGGGCCGTTGTGCTGTCGCAACTCGCCGGCCGCAACGACGTGGTTTTCGGC
GCGACCGCCTCGGGGCGGCCGGCCGACCTGCCCGGTGTGGAGGCGATGGTCGGCCAGCTG
CTCACCACGCTGCCAGTCCGTGTCCGGCTCGACGGCGAGCGGCGGGCGGTCGACCTGTTC
GCCCAGCTGCAGCGCGACCAGTCGGCACTCATGGCTCACCAGCATCTCGGCCTGCAGGAC
GTGCAGGCCGTCGTCGGCCGCGGAGCGGTCTTCGACACGCTCGTCATCTACGAGAACTTC
CCCCGCAAGGGACTCGGCCGGGCGCCGGACGGCGGCCTGACCCTGCTCCCGGTCAAGCGC
GGGCGCAACTCGTCGCACTACCCGTTCACCCTGGTCACCGGTCCAGGCGAGCAGATGCCG
CTCATCCTCGACTACGACCGTGGCCTCTTCGACCCCGCTGCCGCCGAGTCTGTCGTCGGC
GCGTTGGCTCGCGTGCTGGAGCGGCTGGTCGCCGAGCCTGATATCCTCGTCGGCCGTCTG
ACGCTGGTGAGCGAGGCAGAGCGTGCCCTGGTGGTTGACGAATTCAACGCGACCGCGGGC
CCGGTGCCGGGGGAGTCCGTAGTCGAGCTGTTCGCCCACCGGGTGGCCGAAGCGCCGGAC
GCGGTGGCGATCACCGATGCCGGCGGCGCGAACCTGACCTACGCCGAAGTCGACCGGGCG
TCGAACCGGCTGGCCGGCTACCTCGCCGGTCGTGGCGTCGGCCGTGGCGACCGCGTCGGG
GTGGCCATGGACCGCTCGCCGGAGCTGCTGATCGCGTTCCTGGCGATCTGGAAGGCAGGC
GCCGCGTACGTCCCGGTGGACGTCGAGTACCCGGCCGAGCGGATCGCGTTCATTCTCAAC
GACTCCGGGGTCTCGACCGTTCTGTGCACTCAGGACACCAGCGGAGTCGTGCCGGAGAAC
GCGATCGTCCTCGACGCGCCGGACACGCGTGCGTCCATTGAGGACTGTGCGGACACGGCG
CCGGAGATCCGGCTGTACGCAGGCGACCTGGCGTACGTGATGTACACCTCGGGATCCACC
GGCCTGCCGAAGGGCGTGGCCATCCCGCATGGGGCAGTGGCCGGCCTGGCCGGCGACTCG
GGCTGGCAGATCGGTCCCGATGACGGCGTGCTGATGCACGCGACACACGTCTTCGACCCC
TCGCTCTACGCGATGTGGGTGCCGCTCGTCGCGGGTGGCCGGGTCTTGCTCACCGAGCCG
GGCGTGTTGGACGCGGCCGGGGTACGTCAGGCCGTCGAACGGGGTGCGACCGCCGTGCAC
CTCACCGCCGGTTCCTTCCGTGCCCTGGCGGAAACGTCTCCGGAGTGTTTCGCGGGTCTT
GTCGAGATCGGGACCGGCGGAGACGTGGTTCCCGCGCAGTCCGTGGCGAACCTGCGGCGG
GCCCAGCCCGGCCTGCGGGTGCGCAACACCTACGGGCCGACCGAGACCACCCTGTGCGCG
ACGTGGCTGCCGATCGAGCCCGGTGACGTGATCGATCGGGAGCTGCCGATCGGCCACCCG
ATGACCAACCGCAAGATCTACATCCTCGACGCCTTCCTGCGACCGGTCCCGCCGGGAGTG
GCAGGCGAGCTGTACATCGCGGGCACGGGTCTGGCCCGTGGCTACCTCGACAGCCCGGGC
CTGACGGCCGACCGATTCGTGGCGTGCCCGTTCCTCGCCGGTGAACGCATGTACCGCACC
GGTGACGTGGCGCGCTGGACCCGTGATGGTGAGGTGGTGTTCCTCGGCCGCGCCGACGAC
CAGGTGAAGATCCGCGGCTACCGGGTCGAGCTTGGCGAAGTCGAGGCCGTGCTGGCGGCC
CAGCCGGGAGTGGTCGAGGCGGTCGTCATGGCGCGGGAGGACCAGCCCGGTGAGAAGCGC
CTGGTCGGCTACTTCGTGTCCGACGGCAGCGACGCGGGCCCAGCCGAGATCCGCAGGCAA
ATGGCCCAGGTCCTGCCCGACTACATGGTTCCGATCGCGGTCGTCGCCCTGCCCGGCCTG
CCTGTCACACCCAACGGCAAGGTGGATCGCCGGGCCCTGCCCGCCCCGGACCTCGCCGGA
CGCTCGTCGGAGAAAGCGCCTGAGAACGAGACCGAGAAGGTGCTGTGCGCGCTGTTCGGC
GAGATCCTCAGCGTCGACCAGGTGGGCGTCGACGACACCTTCAACGACCTCGGCGGCAGT
TCGGCGCTGGCCATGCGGCTGATCGCGCGGATCCGTGAGGAACTCGGCGCGGATCTGCCC
ATCCGGCAGCTGTTCTCCTCGCCCACACCCGCGGGTCTGGCCAGGGCACTTGCCGCGAAG
TCACGCCCCGCACTGGAAGCCGCCCAGCGGCCGGACCGGGTGCCCCTCACCGCCCGGCAG
CTGCGTGCCTGGCTGCTGGCGCGTCCCGGCGAGGACACTGCCGACCAGCACGTCTCGATC
ACGCTGCGCTTGGGCGGCAAGTTGAACGTGCCCGCGCTGGCGGCGGCGATCGGCGATGTC
GCGGCCCGCCACGAGATCCTCCGCACGACCTTCCCGGGTGACGCGCAGAGCGTTCACCAG
CACGTCCACGACGCCTTGGCAATCGAGCTGACTCCCGTCCCGGCCACCGAGGAGGACCTC
CCCGGGTTGCTCGCCGAGCGGCGTGAGTCGGTGTTCGACCTCACCCAGGAGGTGCCGTGG
CGATGTGACCTTTTCCAGCTCTCGGAAAAGGACCACGTGCTGCAGGTGAAGATCCACCGG
ATCCTCGCTGACGACGACTCGATGGACGTGTTCTTCCGTGACCTGGCGGCCGCGTACGGC
GCGCGGCGTGAAGGCCGGGTCCCTGAGCGTGCGCCACTCGCCCTGCAGTTCGCCGACTTC
GCGATCTGGGAGCACCGGCTTCTCGAGGATGAGCAAGAGCTGGACAGCCTGATCAACGAG
CAGATCGTCTTCTGGCGGGACAACCTCGCCGGTATCGACGGGGAGACGGTGCTCCCGTTC
GACCGCCCGCGCTCGGCTGTCCCGTCGCGGCGGGCGGGCACGGTCGCGCTGCGGCTGGAC
GCCGATCCGCACGCCCGGTTGACGGCAGTGGCAGAGCCGGTAGGCGCGGACACGCTCCAG
ATGGTGCACGCCGCGCTCGCCATGCTGCTGGCCAAGTTCGGAGCCGGCCACGACGTGGTG
ATCGGCACGACGCTGCCGCGGGACGAGGAGCTCTTCGATCTCGAGCCGATGATCGGGCCG
TTCACCCGGCCGCTCGCCCTGCGCACGGACGTCTCGGGCGACCCGACCTTCCTTGAGGTC
GTCACCAGGGTGCAGGAGGCTGCCCAGGCCGCGGGCGAGCACCTGGACGTGCCCTTCGAG
AGGATCGTCGAGCTGCTGGACCTGCCGGCCTCGCTGTCCCGCCACCCGGTGTTCCAGGTG
GGACTGCAGGTGGACGAGGAGGACATCGACGGGTGGGACGCGGCGGAACTGCCCGCCCTG
CGCACCAGCGTCGAACCCGGCGGGACCGAGGCCATGGAGCTGGATCTCGCGGTCAAGCTC
ACCGAGCGCTTCGATGACGACGACAACTCGGGCGGCATCGAGGGTGCGCTGCACTACGCC
ACCGACCTGTTCGATGAGGCCACAGCCGAGTCGGTGGCCCGCCGGCTGGTCCGAGTCCTT
GAACAGGTGGCTGAGGATCCCCAGCGGCGGATCAGCGACCTGGACATCTTCCTGGACGAC
TTCGAACGCGGCCGTCCGGCCATCGCACCGGCGCGGTGGTCCGGGGCCGTACCCGCGGTG
GTCGCCGACCTGGCGCAGGACGGCCCGCTTGGTGCGCTCCTGCTCGACGAGCAGCTGCGC
CCCGTGGCTCCCGGGGCGGTCGGCGATCTTTACGTCACCGGTCCGGCAGTGGACGCGGGA
ACGGCCGGTCTGCCGACCATGCCGTGTCCATTTGGGACTCCCGGGCACCAGATGATGCAC
ACGGGCCTGCTTGCTCGTAAGTCCCACACCAAGGCTTTGGTCGTCGTGGGCGAGCGGCGG
CGATCAAGCGCCTCGGTGAAGACGGGTGACTTCGAGATCCTGCTGCCGCTGCGAGTCGGC
GGTGACCGCCCGCCACTGTTCTGTGTCCACGCGAGCGGTGGCCTGAGTTGGAACTACGAG
CCGTTGCTGCGGTATCTCCCGCCGAATCAACCGGTCTACGGCGTGCAGGCACGCGGCTTG
GCCCGTACCGAACCTTTGCCGGGCAGCGTCGACGAGATGGCGGCCGACTACCTCGAGCAG
ATCCGCGCCGTGCAGCCGACCGGGCCGTACCACCTCCTCGGCTGGTCCCTCGGCGGCAGG
ATCGCGCAGGCGATGGCCAAATTGCTTGAGGCGGAAGGGGAACAGGTCGGCCTGCTCGCT
CTGCTCGACGCGTATCCCGTCTACATGGGACGCAAGACGACCGGCGCCGCGAGCGAGGAA
GAGGCCCTCGAAAGGCGCAACCAGCAGGACTTGGAGCTCGCGGGGCAACTGGTCAAGGGG
GCAGCGGCCCGGTCGCGCCTTGAGGCGGTCATGCGCAACCTCTGGGATGTCGGGCCACGC
CACGTCTCCACGCCGTTCGCCGGCGACATTCTGCTCTTTGTGGCCACTGTGGACCGTCCA
GGGCATCTGCCCGTCCCGGTGGCGAAAGACAGCTGGAAGGACTTCACCAGCGGGGCCATC
GAGGCTCACGAGATCGCGTCCAACCACTACGACATGGTGCAACCCGCGGCGCTGGGCCAG
ATTGGAGCCATCGTCGCCGAGAAACTCCGGTCCCGGCCGGATGCTGAAAGGACACAACGA
TGA
[7] C15..310
[7] A493..889
[7] L-3,5-dihydroxyphenylglycine(Dpg)659..759
[7] PCP967..1038
[7] X1052..1351
[7] TE1624..1841
[7] C43..930
[7] A1477..2667
[7] L-3,5-dihydroxyphenylglycine(Dpg)1975..2277
[7] PCP2899..3114
[7] X3154..4053
[7] TE4870..5523

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[493-889] 4.49999999999996e-108 PF00501
PF00501   AMP-binding
IPR001031 Thioesterase (Domain)
[1624-1841] 4.10000000000004e-37 PF00975
PF00975   Thioesterase
IPR001242 Condensation domain (Domain)
[15-310] 1.5e-64 PF00668 [1052-1351] 1.40000000000001e-47 PF00668
PF00668   Condensation
IPR009081 Acyl carrier protein-like (Domain)
[962-1041] 3.09999999999998e-25 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[967-1038] PS50075
PS50075   ACP_DOMAIN
[964-1039] 9.39998942703831e-19 SSF47336
SSF47336   ACP_like
[974-1037] 3.5e-13 PF00550
PF00550   PP-binding
IPR010071 Amino acid adenylation domain (Domain)
[493-889] 9.10000000000013e-126 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020845 AMP-binding, conserved site (Conserved_site)
[613-624] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
Chlor_00040 Chlor_00040   Chlor_00060 Chlor_00060
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