Chlor_00090 Chlor_00090   Chlor_00110 Chlor_00110

Chlor_00100 : CDS information

close this sectionLocation

Organism
StrainA82846UV37B
Entry nameChloroeremomycin
Contig
Start / Stop / Direction34,245 / 35,258 / + [in whole cluster]
1 / 1,014 / + [in contig]
Location34245..35258 [in whole cluster]
1..1014 [in contig]
TypeCDS
Length1,014 bp (337 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450(fragment)
Product (GenBank)PCZA361.27
GenecepG
oxyC
ORF9
Gene (GenBank)
EC number
Keyword
  • 3rd cross-linking
  • AB ring
Note
  • C-terminal sequence of Chlor_00090(nt.34133-) overlap with N-terminus of Chlor_00100.
Note (GenBank)
  • similar to P450 related oxidase/hydroxylase
Reference
ACC
PmId
[9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998)
[12596194] Vancomycin assembly: nature's way. (Angew Chem Int Ed Engl. , 2003)
comment
[PMID: 9545426](1998)
chloroeremomycin生合成 gene clusterの報告。

ORF9(450aa): P450-related oxidase
配列解析のみ。ion-heme binding motifを含んでいる。

---
[PMID: 12596194](2003)
グリコペプチド系抗生物質の生合成に関するレビュー。

chloroeremomycin-producing (Cep) A. orientalis由来約40genesのうち、明らかにグリコペプチド生合成経路に割り当てられる30genesを、タンパク名、予測された or 知られた機能、酵素活性の補助基質・補因子などと共にリスト化している。

>Orf9
metabolic pathway : oxidative cross-linking
protein name : CepG (455aa)
function : oxidation
cofactor : NADPH
クローニングと精製はされたことがあるが、解析はされていない。
Related Reference
ACC
O87675
NITE
Balhi_00130
PmId
[12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001)
[16730832] Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. (J Biotechnol. , 2006)
comment
BLAST id95%, C末側にhit
Amycolatopsis balhimycina_oxyC
P450 monooxygenase

---
[PMID: 12404385](2001)
oxyAと同様にoxyB, oxyCのmutantを作製し、その産物の構造から各oxygenaseの役割と順番を割り当てている。

1) OxyBによるC-O-D ring systemの形成
2) OxyAによるD-O-E ring systemの形成
3) OxyCによるbiaryl AB systemの形成

また、bicyclic C-O-D-O-E ring中間体は、1LeuのN-methylationと、4HpgのO-glycosylationの基質となれることもわかった。

---
[PMID: 16730832](2006)
oxyA, oxyB, oxyCの非極性mutantsを作製し、閉環反応の順番を再確認している。
また、これらmutant株をvancomycin産生菌A. orientalis DSM40040由来の対応するoxygenase genesで補完すると、balhimycin産生は回復した。

close this sectionSequence

selected fasta
>putative cytochrome P450(fragment) [PCZA361.27]
DHENFTTRPQFTHAKSDAHVEAQFVGQISTYDPPEHTRLRKMLTPEFTVRRIRRMEPAIQ
GLIDDRLDMVEAEGPPADLQGLFADPVGALALCELLGIPRDDQREFVRRIRRNTDLSRGL
KARAADSAAFNRYLDNLISRQRKDPDEGFLGMIVREHGDNVTDEELKGLCTALILGGVET
VAGMIGFGVLALLENPDQIQLLFAGPEKADRVVNELVRYLSPVQAPNPRLAIKDVVIDGQ
LIKAGDYVLCSVLMANRDEALTPNPNVLDANRAAVSDVGFGHGIHYCVGAALARSMLRMA
YQTLWQRFPGLRLAVPIAEVKYRSAFVDCPDQVPVTW
selected fasta
>putative cytochrome P450(fragment) [PCZA361.27]
GATCACGAGAATTTCACGACGCGGCCGCAATTCACCCATGCGAAATCTGACGCACACGTA
GAGGCCCAGTTCGTCGGACAGATATCGACTTACGATCCGCCTGAGCACACTCGCCTGCGA
AAGATGCTGACGCCGGAGTTCACGGTGCGGCGGATTCGCCGGATGGAGCCCGCGATTCAG
GGCCTCATCGATGATCGCCTCGACATGGTGGAGGCCGAGGGGCCGCCCGCGGATCTCCAA
GGGTTGTTCGCCGACCCGGTCGGCGCTCTCGCCCTGTGTGAACTGCTCGGCATTCCGCGC
GATGACCAGCGTGAGTTCGTCCGGAGGATCAGGCGGAACACCGATCTGAGCCGGGGACTC
AAGGCACGGGCGGCCGACAGCGCGGCGTTCAACCGGTACCTGGACAACCTCATCTCCCGG
CAGCGCAAGGACCCCGACGAGGGGTTTCTCGGCATGATCGTGCGAGAGCACGGTGACAAC
GTCACCGACGAGGAACTGAAGGGCCTGTGCACGGCGCTGATCCTCGGCGGTGTCGAGACC
GTCGCGGGGATGATCGGCTTCGGAGTGCTCGCCCTGCTGGAGAATCCTGACCAGATCCAG
TTGCTTTTCGCCGGCCCCGAGAAGGCCGACCGCGTCGTCAACGAGCTGGTGCGTTATCTG
TCCCCGGTGCAGGCGCCGAATCCACGCCTCGCCATCAAGGACGTCGTCATCGACGGACAG
TTGATCAAGGCAGGTGATTACGTCCTGTGCTCGGTTCTCATGGCCAACCGAGATGAAGCG
CTGACGCCGAATCCCAACGTTCTCGACGCGAATCGCGCCGCGGTGTCGGACGTCGGATTC
GGGCACGGCATCCATTACTGCGTGGGCGCGGCGCTGGCCCGGTCGATGCTGCGGATGGCT
TATCAGACCCTGTGGCAGCGATTTCCCGGGCTGCGGCTGGCAGTGCCGATCGCGGAAGTG
AAGTACCGCAGTGCGTTCGTCGACTGCCCTGATCAGGTTCCAGTCACCTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[2-337] 4.49999999999996e-84 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[1-337] 1.59998835313644e-84 SSF48264
SSF48264   Cytochrome_P450
[210-308] 7.5e-13 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[31-42] 1.49999648196323e-46 PR00359 [78-94] 1.49999648196323e-46 PR00359 [95-110] 1.49999648196323e-46 PR00359 [132-154] 1.49999648196323e-46 PR00359 [211-222] 1.49999648196323e-46 PR00359 [229-256] 1.49999648196323e-46 PR00359 [257-272] 1.49999648196323e-46 PR00359 [278-287] 1.49999648196323e-46 PR00359 [287-298] 1.49999648196323e-46 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[280-289] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Chlor_00090 Chlor_00090   Chlor_00110 Chlor_00110
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