Chlor_00230 Chlor_00230   Chlor_00250 Chlor_00250

Chlor_00240 : CDS information

close this sectionLocation

Organism
StrainA82846UV37B
Entry nameChloroeremomycin
Contig
Start / Stop / Direction51,344 / 52,759 / + [in whole cluster]
17,100 / 18,515 / + [in contig]
Location51344..52759 [in whole cluster]
17100..18515 [in contig]
TypeCDS
Length1,416 bp (471 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase
Product (GenBank)PCZA361.3
GeneevaA
ORF23
Gene (GenBank)
EC number
Keyword
  • L-4-epivancosamine
Note
Note (GenBank)
  • similar to sugar biosynthesis gene
Reference
ACC
PmId
[9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998)
[11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000)
[23473392] Structure of EvaA: a paradigm for sugar 2,3-dehydratases. (Biochemistry. , 2013)
comment
[PMID: 9545426](1998)
chloroeremomycin生合成 gene clusterの報告。

ORF23(471aa): 2,3 Dehydratase
配列解析のみ。NDP-4-epi-vancosamineの生合成への関与が割り当てられている。

---
[PMID: 11035791](2000)
dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。

ORF23はdTDP-6-deoxy-4-keto-D-glucoseを基質として、2,6-dideoxy-3,4-diketo-dTDP sugarの分解化合物をもたらした。ORF23とORF25の組み合わせは、C-3アミノ基がequatorialなdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseをもたらした。

すべての結果から提唱されている経路は次の通り。

dTDP-4-keto-6-deoxy-D-glucose

↓ ORF23(EvaA): C-2 deoxygenation

↓ ORF25(EvaB): C-3 amination

dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose

↓ ORF14(EvaC): C-3 methylation

dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose

↓ ORF26(EvaD): C-5 epimerization

↓ ORF24(EvaE): C-4 ketoreduction

dTDP-L-epivancosamine

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[PMID: 23473392](2013) 本文未確認。
EvaAの構造解析。

EvaAは2量体で、2つのdTDP-sugar binding pockets A and Bが各subunitに存在する。site-directed mutagenesis実験と活性分析に基づき、pocket Aがactive siteとして機能し、pocket Bは遺伝子複製現象の残遺物であると思われる。

close this sectionSequence

selected fasta
>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [PCZA361.3]
MSSFVVPSLTAVRPRDHHDYADRIALSAATTDGVQMRTEDVRAWIAERRDANVFHVERIP
FADLDQWWFEGVTGNLVHRSGRFFTIEGLHVIEHDGPHGDGPYREWQQPVIRQPEVGILG
ILAKEFDGVLHFLMQAKMEPGNPNLVQLSPTVQATRSNYTKAHGGTNVKLIEYFAPPDPE
RVIVDVLQAEQGSWFFRKSNRNMIVETVDDVPLWDDFCWLTLGQIAELMHEDETINMNSR
SVLSCLPYQDITPRALFSDVQLLSWFTNERSRHDVRVRRIPLADVCGWKQGAEEIEHEDG
RYFKVLAVAVKGSNREKISWTQPLVESVDLGVVAFLVRKIDGVPHVLVQARVDGGFLDTV
ELAPTVQCTPLNYAHLPAEERPPFLDLVQNAPRSRIRYEAIHSEEGGRFLGVRARYLVID
ADEAIDPPPGYAWVTPAQLTALTRHGHYVNVEARTLLACINAAAAQPRGGA
selected fasta
>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [PCZA361.3]
ATGTCGTCCTTCGTCGTTCCATCACTCACCGCGGTGCGCCCTCGTGACCACCACGACTAC
GCCGACCGCATCGCCTTGTCGGCCGCGACCACCGATGGGGTACAGATGCGGACCGAGGAT
GTCCGCGCCTGGATCGCCGAACGCCGCGACGCCAACGTCTTCCACGTCGAACGAATCCCG
TTCGCCGACCTGGACCAGTGGTGGTTCGAAGGCGTGACCGGCAATCTGGTGCACCGCAGC
GGGCGGTTCTTCACCATCGAGGGCCTGCACGTGATCGAGCACGACGGCCCGCACGGCGAC
GGCCCGTACCGCGAGTGGCAGCAGCCGGTCATCAGGCAGCCCGAAGTGGGCATCCTCGGC
ATCCTGGCCAAGGAGTTCGACGGGGTGCTGCACTTCCTGATGCAGGCCAAGATGGAGCCG
GGCAACCCCAACCTGGTGCAGCTCTCGCCGACCGTGCAGGCCACCCGGAGCAACTACACC
AAGGCGCACGGCGGTACGAACGTCAAGCTGATCGAGTACTTCGCGCCGCCGGACCCCGAG
CGGGTCATCGTCGACGTTCTCCAGGCGGAGCAAGGATCCTGGTTCTTCCGCAAGTCCAAT
CGCAACATGATCGTCGAGACCGTCGACGACGTGCCGTTGTGGGACGACTTCTGCTGGCTC
ACCCTCGGCCAGATCGCCGAACTGATGCACGAGGACGAGACGATCAACATGAACTCCCGA
AGCGTGTTGTCGTGTCTGCCCTACCAGGACATAACTCCTCGCGCGCTGTTCTCCGACGTT
CAGCTCCTGTCGTGGTTCACCAACGAGCGTTCGCGTCACGATGTGCGCGTCCGCCGCATT
CCGCTCGCGGACGTGTGCGGCTGGAAGCAAGGCGCCGAGGAGATCGAGCACGAGGACGGC
CGTTACTTCAAGGTCCTCGCGGTCGCCGTGAAAGGCAGCAACCGCGAGAAGATCAGTTGG
ACCCAGCCGTTGGTCGAATCCGTCGACTTGGGTGTCGTCGCGTTCCTCGTGCGGAAGATC
GACGGTGTGCCGCACGTTCTGGTGCAGGCCCGCGTTGACGGTGGTTTCCTGGACACGGTC
GAGTTGGCGCCGACCGTCCAGTGCACGCCCCTCAACTACGCACACCTGCCCGCGGAGGAG
CGTCCGCCGTTCCTTGACCTCGTCCAGAACGCACCCCGGTCGCGAATCCGTTACGAGGCG
ATCCATTCCGAAGAAGGCGGCCGCTTCCTCGGCGTCCGGGCCCGATATCTCGTGATCGAC
GCGGACGAGGCAATCGATCCACCGCCTGGCTACGCCTGGGTCACCCCGGCGCAGCTCACC
GCGCTCACCCGGCACGGGCACTACGTCAACGTCGAGGCTCGCACGCTTCTCGCGTGCATC
AACGCCGCGGCGGCCCAGCCTCGGGGAGGTGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005212 NDP-hexose 2,3-dehydratase (Domain)
[37-246] 3.49999999999995e-82 PF03559 [259-460] 1.5e-83 PF03559
PF03559   Hexose_dehydrat
SignalP No significant hit
TMHMM No significant hit
Chlor_00230 Chlor_00230   Chlor_00250 Chlor_00250
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