Chlor_00240 Chlor_00240   Chlor_00260 Chlor_00260

Chlor_00250 : CDS information

close this sectionLocation

Organism
StrainA82846UV37B
Entry nameChloroeremomycin
Contig
Start / Stop / Direction52,761 / 53,738 / + [in whole cluster]
18,517 / 19,494 / + [in contig]
Location52761..53738 [in whole cluster]
18517..19494 [in contig]
TypeCDS
Length978 bp (325 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-hexose C-4 ketoreductase
Product (GenBank)PCZA361.4
GeneevaE
ORF24
Gene (GenBank)
EC number
Keyword
  • L-4-epivancosamine
  • equatorial-OH
Note
Note (GenBank)
  • similar to dTDP-dehydrogenase
Reference
ACC
PmId
[9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998)
[11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000)
comment
[PMID: 9545426](1998)
chloroeremomycin生合成 gene clusterの報告。

ORF24(325aa): 4-Ketoreductase
配列解析のみ。NDP-4-epi-vancosamineの生合成への関与が割り当てられている。

---
[PMID: 11035791](2000)
dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。

ORF26でdTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-uloseを反応させると、分子量は変わらないがchromatographyだと明らかに異なる化合物をもたらしたことから、ORF26はC-5 epimeraseとして機能すると予測された。C-5での重水素の取り込みで、これを確認している。
さらにORF24と補助基質NADPHを追加して反応させると、dTDP-L-epivancosamineが得られた。

すべての結果から提唱されている経路は次の通り。

dTDP-4-keto-6-deoxy-D-glucose

↓ ORF23(EvaA): C-2 deoxygenation

↓ ORF25(EvaB): C-3 amination

dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose

↓ ORF14(EvaC): C-3 methylation

dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose

↓ ORF26(EvaD): C-5 epimerization

↓ ORF24(EvaE): C-4 ketoreduction

dTDP-L-epivancosamine

close this sectionSequence

selected fasta
>dTDP-hexose C-4 ketoreductase [PCZA361.4]
MKLITVLGASGFIGSAVTRALAQQPIRLRAVARRQFTPAPGQAETTVVAADLTDRVALAD
AVAGSDAVVYLLLSDGGWRAVETEDAERVNVGVMRDLIDVTGSDNGTPPVVVFGGTVSQV
GVPPREPLDGSEPDNPATPYDIQKLTAEQILKKATANGQVRGISLRLPTIFGETTAQGAN
HDRGVVSSMARRALDGQALTIWGDGSVRRDVVHVEDVAAAFTAALANPDSLVGGHWLIGA
GRGDQLGEIFRLVAREVAEQTGQRPVEVTCVEPPSHAPEMDFRSVTIDSSPFRAVTGWRP
EISLSEGVRRTVAALTTSVHGKARA
selected fasta
>dTDP-hexose C-4 ketoreductase [PCZA361.4]
ATGAAGCTGATCACCGTGCTCGGTGCGTCGGGCTTCATCGGCTCGGCTGTCACGCGTGCA
CTGGCGCAGCAGCCAATCCGGCTGCGAGCGGTGGCGCGCAGGCAGTTCACGCCCGCGCCC
GGCCAAGCCGAGACGACCGTCGTCGCCGCTGATCTCACCGACCGTGTCGCGCTCGCCGAC
GCGGTCGCGGGATCGGACGCGGTCGTGTACCTGCTTCTGTCAGACGGCGGATGGCGCGCG
GTCGAGACCGAGGACGCCGAACGCGTGAACGTGGGCGTCATGCGGGACCTCATCGACGTC
ACCGGCAGCGACAACGGGACGCCCCCGGTGGTGGTGTTCGGCGGTACCGTCTCGCAGGTC
GGTGTGCCACCTCGGGAGCCGCTCGACGGCAGCGAGCCCGACAACCCGGCGACTCCCTAC
GACATACAGAAGCTGACAGCGGAACAGATCCTCAAGAAGGCCACGGCAAATGGCCAGGTG
CGCGGCATCAGCCTGCGTCTGCCGACGATATTCGGTGAAACCACGGCACAAGGCGCGAAC
CACGACCGCGGTGTCGTGTCGTCCATGGCGCGGCGAGCGCTCGACGGCCAGGCACTCACC
ATCTGGGGCGACGGCAGCGTGCGACGCGACGTCGTCCATGTCGAGGACGTCGCGGCGGCG
TTCACCGCGGCACTGGCCAACCCGGATTCCCTTGTCGGCGGCCACTGGCTGATCGGCGCG
GGCCGAGGCGATCAGCTTGGGGAGATTTTCCGCCTCGTGGCACGGGAAGTGGCCGAGCAG
ACCGGGCAGCGCCCGGTCGAGGTGACCTGTGTGGAACCACCGTCGCACGCACCTGAGATG
GATTTCCGCAGCGTCACCATCGATTCCTCGCCGTTCCGGGCGGTCACCGGCTGGCGCCCA
GAGATTTCGCTGTCCGAAGGAGTGCGTCGCACTGTCGCCGCATTGACGACATCAGTTCAT
GGAAAGGCTCGCGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[4-239] 1.6e-48 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[4-191] 5.69999999999998e-24 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Chlor_00240 Chlor_00240   Chlor_00260 Chlor_00260
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