Chlor_00310 Chlor_00310   Chlor_00330 Chlor_00330

Chlor_00320 : CDS information

close this sectionLocation

Organism
StrainA82846UV37B
Entry nameChloroeremomycin
Contig
Start / Stop / Direction59,813 / 60,901 / + [in whole cluster]
25,569 / 26,657 / + [in contig]
Location59813..60901 [in whole cluster]
25569..26657 [in contig]
TypeCDS
Length1,089 bp (362 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative phospho-2-dehydro-3-deoxyheptonate aldolase
putative 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
putative DAHP synthase
Product (GenBank)PCZA361.10
Gene
Gene (GenBank)
EC number2.5.1.54
Keyword
  • Bht
  • Hpg
  • Dpg
Note
Note (GenBank)
  • similar to phospho-2-dehydro-3-deoxyheptonate aldolase
Reference
ACC
PmId
[9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998)
comment
chloroeremomycin生合成 gene clusterの報告。

ORF31(362aa): Phospho-2-dehydro-3-Deoxyheptonate aldolase
配列解析のみ。詳細記載なし。
Related Reference
ACC
Q799B0
NITE
Balhi_00360
PmId
[20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010)
comment
BLAST id85%
Amycolatopsis balhimycina_ald
Phospho-2-dehydro-3-deoxyheptonate aldolase(EC:2.5.1.54)

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。

balhimycinのaglycone骨格となるアミノ酸(Bht, Hpg, Dpg)は、直接的または間接的にshikimate経路に由来する。shikimate経路の律速酵素Dahp synthaseをコードするdahpの追加コピーを発現した株は、グリコペプチド生産性が3倍に改善された。逆にdahpのdeletion mutant株はbalhimycin産生で有意な減少をもたらした。
ACC
Q6TZ04
PmId
comment
BLAST id60%
Amycolatopsis methanolica_aroF
Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)

DAHP-synthaseを2つ(aroG, aroF)もっている。それぞれを発現させ、活性をみたり、何により阻害されるかを確認している。
aroG(DS1:plant-type DAHP synthase[Q6TZ03])
aroF(DS2:E. coli-type DAHP synthase[Q6TZ04])
ACC
P0AB91
PmId
[1682314] ( , )
[8778789] ( , )
[12126632] ( , )
comment
BLAST id54%
Escherichia coli (strain K12)_aroG
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (EC:2.5.1.54)

close this sectionSequence

selected fasta
>putative phospho-2-dehydro-3-deoxyheptonate aldolase [PCZA361.10]
MTTSTPAAAVDLDNQRIDRIVPLVTPALLHHELPLSAKAADTVRRGRENVLRVLDGTDDR
LLVITGPCSIHDPAAALDYADHLATMAGRFTDDLLIVMRVYFEKPRTVGGWKGLINDPHL
DGTGDVNHGLRIARHLLLELAERGLPVACEWLDTTIPAYLADTVSWGAIGARTVESQNHR
MLASGLSMPVGFKNRRDGDITVAIDAIRAAAARHVVPGVDPGGLPAILHTAGNPDCHLVL
RGGNSAPNHDASSVHTALTALQDAGLPRRVVIDASHDNSRKDHHRQPVVAGQIADQVKGG
QRGIVGVMLESNLRAGRQDLRPGQPLTYGQSITDACIDIPTTQTVLQTLATATAARRRLS
QN
selected fasta
>putative phospho-2-dehydro-3-deoxyheptonate aldolase [PCZA361.10]
ATGACAACATCTACTCCCGCCGCCGCGGTCGACCTCGACAACCAGAGGATCGACCGCATA
GTCCCATTGGTGACACCGGCCCTGCTGCACCATGAACTGCCGCTCAGCGCCAAGGCAGCC
GACACCGTGCGACGAGGTCGTGAGAACGTCCTTCGCGTCCTCGACGGTACGGATGACCGG
CTGCTCGTGATCACTGGACCGTGCTCCATCCACGACCCGGCCGCGGCCCTCGACTACGCC
GACCACCTCGCCACGATGGCCGGCCGGTTCACTGACGACCTCCTGATCGTCATGCGCGTG
TACTTCGAGAAACCGCGAACGGTCGGTGGCTGGAAAGGGCTCATCAACGACCCCCACCTC
GACGGCACCGGCGACGTGAACCACGGCCTGCGAATCGCCCGGCACCTGTTGCTGGAGCTC
GCCGAACGCGGCCTGCCCGTCGCGTGCGAGTGGCTGGACACGACCATCCCGGCCTACCTC
GCCGACACCGTTTCCTGGGGCGCGATCGGTGCCCGCACCGTGGAAAGCCAAAACCACCGC
ATGCTCGCCAGCGGTCTGTCCATGCCGGTCGGCTTCAAGAACCGCCGCGACGGCGACATC
ACCGTGGCCATCGACGCGATCCGCGCTGCGGCGGCCCGTCACGTCGTTCCTGGCGTCGAC
CCCGGCGGCTTGCCCGCCATCCTGCACACCGCCGGCAACCCCGACTGCCATCTTGTTCTG
CGCGGCGGCAATTCCGCGCCCAACCACGACGCCTCGTCTGTCCACACAGCACTCACCGCG
CTGCAGGACGCCGGTCTTCCCCGCCGGGTCGTGATCGATGCCAGCCATGACAACAGCCGC
AAGGACCACCACCGGCAACCTGTTGTCGCCGGGCAGATCGCCGACCAGGTCAAGGGCGGC
CAACGCGGCATTGTTGGCGTGATGCTCGAATCCAACCTCCGCGCCGGCCGCCAGGACCTC
CGCCCCGGCCAGCCTCTGACCTACGGCCAGTCCATCACGGACGCCTGCATCGACATCCCC
ACGACGCAGACCGTTCTGCAGACCCTCGCCACGGCAACCGCGGCTCGGCGGCGTCTCAGC
CAGAACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006218 DAHP synthetase I/KDSA (Domain)
[52-346] 2.20000000000002e-84 PF00793
PF00793   DAHP_synth_1
IPR006219 DHAP synthase, class 1 (Family)
[1-361] PIRSF001361
PIRSF001361   DAHP_synthase
[13-357] TIGR00034
TIGR00034   AroFGH
IPR013785 Aldolase-type TIM barrel (Domain)
[16-358] G3DSA:3.20.20.70
G3DSA:3.20.20.70   Aldolase_TIM
SignalP No significant hit
TMHMM No significant hit
Chlor_00310 Chlor_00310   Chlor_00330 Chlor_00330
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