Lydi_00200 Lydi_00200   Lydi_00220 Lydi_00220

Lydi_00210 : CDS information

close this sectionLocation

Organism
StrainTP-A0598 (=NBRC 110027)
Entry nameLydicamycin
Contig
Start / Stop / Direction110,570 / 109,281 / - [in whole cluster]
110,570 / 109,281 / - [in contig]
Locationcomplement(109281..110570) [in whole cluster]
complement(109281..110570) [in contig]
TypeCDS
Length1,290 bp (429 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)cytochrome P450
GeneTPA0598_03_00850
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[26380643] Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a producer of anti-MRSA antibiotic lydicamycins. (Stand Genomic Sci. , 2015)
comment
Streptomyces sp. TP-A0598におけるputative lydicamycin biosynthetic gene clusterの報告。
このclusterが実際にlydicamycin産生に関わるかどうかの実験的な確認はされていない。

TPA0598_03_00850(429aa): cytochrome P450

C-8でのoctalin炭素のhydroxylationが割り当てられている。配列解析のみ。
Related Reference
ACC
Q156A0
PmId
comment
BLAST id47%
Amycolatopsis orientalis_ebh
Epothilone b hydroxylase

epothilonesはepothilone A, BがS. cellulosumにおける主な産物であるが、同時に関連化合物が37も分離されている。関連化合物のひとつepothilone Fは、S. cellulosum, Amycolatopsis orientalis, Amycolata autotrophicaにepothilone Bを加えることで形成される。

Amycolatopsis orientalisにおいてcytochrome P450を2つ同定、クローニングし、Streptomyces lividansで発現。ebhを発現したtransformantはepothilone B → epothilone F へとC-21 hydroxylationできるが、もうひとつのcytochrome P450とコントロールではそれができない。

close this sectionSequence

selected fasta
>putative cytochrome P450 [cytochrome P450]
MTEIDSNGSVRELNFPMSRRCPFAPPPEYERLRADSPLVRVQLPHGGTAWVVTRHAEAQQ
LLTDPRVSSDASRPGFPSVRPPQEPPADEGTDGAEPLTAEQLTELAEIAQFHEGQFINLD
PPRHGHYRRMLISEFGARRMNELRPEVQRITDRLVDDMLAKGSPADLVEDLGLPLSSLVI
CRLLGVPYEDHEYFQSATRTLVQLDAEPEQVKAAMEGLIDFLDELVKAAVRAPGDNLIGR
LINEQVGSGELTRAALVGMAFLLLTAGHEATANMLPLGMLTLLEHPQQLAELRENPALLP
GAVDELLRFHSVSDWAAFDRVAVEDIEIDGRKIMAGEGIFVLSASANRDERVFERPDTFD
IHRKARHHIAFGYGVHQCIGQNLARAELEIGLGTLLRRIPALRLDKPMDELPFKYQNFVF
GLHAMPVRW
selected fasta
>putative cytochrome P450 [cytochrome P450]
ATGACGGAGATTGACAGCAACGGGTCGGTGCGGGAATTGAACTTCCCCATGTCCCGGCGC
TGCCCCTTCGCCCCGCCACCGGAGTACGAAAGGCTGCGAGCGGACTCCCCGCTGGTGCGG
GTGCAGCTGCCGCACGGCGGTACCGCGTGGGTGGTGACCCGGCACGCGGAGGCCCAGCAG
CTGCTGACCGACCCACGGGTCAGCTCGGATGCGAGCCGGCCGGGCTTCCCCAGCGTCCGG
CCGCCCCAGGAGCCGCCGGCCGACGAGGGCACGGACGGGGCGGAACCGCTCACCGCCGAG
CAGCTCACCGAGCTGGCGGAGATCGCCCAGTTCCACGAGGGCCAGTTCATCAACCTGGAT
CCGCCCCGGCACGGTCACTACCGGCGCATGCTCATCTCCGAGTTCGGTGCGCGGCGGATG
AACGAGCTGCGGCCGGAGGTCCAGCGCATCACCGACCGGCTGGTCGACGACATGCTGGCG
AAGGGCTCCCCGGCGGACCTCGTGGAGGATCTGGGGCTGCCGCTCTCCTCGCTGGTCATC
TGCCGGTTACTCGGCGTCCCGTACGAGGATCACGAGTACTTCCAGTCCGCGACCAGGACA
CTGGTCCAGCTGGACGCCGAGCCCGAGCAGGTCAAGGCGGCGATGGAGGGGCTCATCGAC
TTCCTCGACGAGCTGGTCAAGGCGGCCGTGCGGGCACCCGGCGACAACCTCATCGGCCGG
CTGATCAACGAACAGGTCGGCAGCGGTGAGCTGACCCGTGCGGCGCTGGTGGGCATGGCC
TTCCTGCTGCTGACCGCCGGACACGAGGCGACCGCGAACATGCTTCCGCTGGGCATGCTG
ACCCTGCTGGAGCATCCGCAGCAGCTGGCCGAACTCCGCGAGAACCCCGCGCTGCTGCCC
GGCGCCGTCGATGAGCTGCTGCGGTTTCACTCCGTGTCGGACTGGGCGGCCTTTGACCGG
GTCGCCGTTGAGGACATCGAGATCGACGGCCGGAAAATCATGGCCGGTGAGGGCATTTTC
GTGCTGAGCGCGTCGGCCAACCGGGACGAGCGGGTCTTCGAGCGCCCCGATACGTTCGAC
ATCCACCGCAAGGCCCGGCACCACATCGCGTTCGGGTACGGGGTGCACCAGTGCATCGGG
CAGAATCTGGCCCGCGCCGAGTTGGAGATCGGTCTGGGCACGCTGCTGCGGCGGATTCCC
GCTCTGCGGCTCGACAAGCCGATGGATGAACTTCCGTTCAAATACCAGAACTTCGTGTTC
GGACTGCACGCGATGCCGGTCCGCTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[266-283] 2.5e-07 PR00385 [301-312] 2.5e-07 PR00385 [369-378] 2.5e-07 PR00385 [378-389] 2.5e-07 PR00385
PR00385   P450
[16-429] 1.7e-105 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
[214-398] 3.4e-26 PF00067
PF00067   p450
[11-429] 6.7e-95 SSF48264
SSF48264   Cytochrome P450
IPR002397 Cytochrome P450, B-class (Family)
[119-130] 2e-52 PR00359 [166-182] 2e-52 PR00359 [183-198] 2e-52 PR00359 [220-242] 2e-52 PR00359 [301-312] 2e-52 PR00359 [320-347] 2e-52 PR00359 [348-363] 2e-52 PR00359 [369-378] 2e-52 PR00359 [378-389] 2e-52 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[371-380] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Lydi_00200 Lydi_00200   Lydi_00220 Lydi_00220
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