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Medium Detail Information

Medium No.
1649
Medium
XYP medium
Composition
Solution A*
  Bacto Yeast Extract (Difco) 10 g
  Hipolypepton** 5 g
  Sodium acetate 2 g
  Tween 80 solution*** 10 ml
  Salts solution**** 5 ml
  Ager (if needed) 15 g
  Distilled water 900 ml
  pH 6.8  
Solution B*
  Xylose 10 g
  Distilled water 100 ml
             
    *Solutions A and B are autoclaved separately. After the solutions are cooled down at 50-60 °C, mixed each other.
             
    **Wako Pure Chemical Ind., Ltd., Osaka, Japan.
             
    ***Tween 80 solution
      Tween 80 50 mg
      Distilled Water 1 ml
             
    ****Salts solution
      MgSO4·7H2O 40 mg
      MnSO4·4H2O 2 mg
      FeSO4·7H2O 2 mg
      NaCl 2 mg
      Distilled Water 1 ml
    Add a drop of 12 N HCl to 500 ml of the salts solution.
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