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Medium Detail Information

Medium No.
Modified Pfennig's Medium II (green sulfur bacteria)
 Vitamin B12 (0.002%) solution1ml
 Trace element solution SL-10B*1ml
 Distilled water998ml
 pH 6.8 
  Dissolve ingredients except Vitamin B12 and sulfide, dispense in culture vessels, and autoclave under a N2/CO2 atmosphere (80/20). Add vitamin and sulfide** from sterile anaerobic stock solutions. The vitamin solution should be sterilized by filtration. Adjust the pH of the medium with sterile HCl or Na2CO3 solutions (2 mol/L each) to pH 6.8.
  *Trace element solution SL-10B
   HCl (25%)7.7ml
   Distilled water1L
  **Neutralized sulfide solution
   Distilled water100ml
  The sulfide solution is prepared in a vessel sealed with a butyl rubber stopper. Autoclave at 121℃ for 15 min under a N2 atmosphere. After cooling to room temperature, the pH is adjusted to about 7.3 by adding of sterile 2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow colour indicates the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.