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Medium Detail Information

Medium No.
Methanofollis ethanolicus medium
 Yeast extract0.1g
 Sodium acetate0.82g
 Vitamin solution*2ml
 Trace element solution**2ml
 Se/W solution***1ml
 Resazurin solution****1ml
 Distilled water1L
  For vial cultures, dissolve all ingredients except for ethanol, vitamin solution, Na2S·9H2O and cysteine-HCl, flush N2/CO2 (80/20) and autoclave. Concentrated Na2S·9H2O and cysteine-HCl solutions are sterilized separately by autoclaving under a N2 atmosphere in tightly closed vessels. Add filter-sterilized 0.8 M ethanol and vitamin solutions, and autoclaved Na2S·9H2O and cysteine-HCl solutions . Inoculum size 10-20%.
  *Vitamin solution
   Folic acid0.9mg
   Nicotinic acid0.2mg
   Vitamin B122.7mg
   p-Aminobenzoic acid0.3mg
   Lipoic acid0.4mg
   Distilled water1L
  **Trace element solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve NTA in 200 ml of water and adjust the pH to 6.5 with KOH.
  ***Se/W solution
   Distilled water1L
  ****Resazurin solution
   Distilled water1L