National Institute of Technology and Evaluation
NITE Biological Resource Center
Biotechnology Center
HOME > NBRC > NBRC Culture Collection > NBRC Culture Catalogue Search > Catalogue Search Result

Medium Detail Information

Medium No.
Thermoheterotroph medium 
 Bacto Yeast Extract (Difco)5g
 Vitamin solution*2ml
 Trace element solution**1ml
 Distilled water1L
  Mix ingredients except for Na2CO3, vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 3% solutions under a N2 atmosphere. Prior to inoculation, add the filter-sterile 5% Na2CO3 and vitamin solutions, cysteine-HCl, and Na2S·9H2O.
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.

contact us
Disclaimer Terms of use
National Institute of Technology and Evaluation (JCN 9011005001123)
2-49-10 Nishihara, Shibuya-ku, Tokyo, Japan