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Medium Detail Information

Medium No.
1039 
Medium
Thermoheterotroph medium 
Composition
 K2HPO40.05g
 KH2PO40.09g
 NH4Cl0.25g
 MgSO4·7H2O0.25g
 CaCl2·2H2O0.15g
 Bacto Yeast Extract (Difco)5g
 Na2S2O3·5H2O2.5g
 Na2CO30.25g
 Vitamin solution*2ml
 Trace element solution**1ml
 Resazurin1mg
 Cysteine-HCl0.3g
 Na2S·9H2O0.3g
 Distilled water1L
  Mix ingredients except for Na2CO3, vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 3% solutions under a N2 atmosphere. Prior to inoculation, add the filter-sterile 5% Na2CO3 and vitamin solutions, cysteine-HCl, and Na2S·9H2O.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
 
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