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Medium Detail Information

Medium No.	1065
Medium	Medium for strain JA363, JA395 and JA418
Composition
KH2PO4 0.5 g   MgSO4·7H2O 2 g   NaCl 20 g   NH4Cl 0.6 g   CaCl2·2H2O 0.15 g   Sodium pyruvate 3 g   NaHCO3 (10% (w/v)) 10 ml   Yeast extract 0.3 g   Peptone 0.3 g   Ferric citrate solution* (0.1%) 5 ml   Trace element solution SL7** 1 ml   Na2S·9H2O 0.24 g   Resazurin 1 mg   Distilled water 1 L   pH 8       Mix ingredients except for NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them.                   *Soluble in hot water.                   **Trace element solution SL7       HCl (25%; 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 60 mg       CoCl2·6H2O 200 mg       CuCl2·2H2O 20 mg       NiCl2·6H2O 20 mg       Na2MoO4·2H2O 40 mg       Distilled water 990 ml     Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
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