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Medium Detail Information

Medium No.
 Yeast extract1g
 Bacto Tryptone (Difco)1g
 Wolfe's modified mineral solution*50ml
 Vitamin mixture***10ml
 Distilled water939ml
 pH 6.5 
  Dissolve ingredients except for vitamin, cysteine-HCl and sulfide, dispense under a N2 atmosphere in culture vessels and autoclave. Add vitamin, cysteine-HCl and sulfide from sterile anaerobic stock solutions prepared under a N2 atmosphere. The vitamin and cysteine-HCl solutions should be sterilized by filtration. Adjust the pH of the medium with sterile 10 N KOH solution to pH 6.5.
  *Wolfe's modified mineral solution
   Nitrilotriacetic acid (NTA)1.5g
   Boric acid0.01g
  Nitrilotriacetic acid is suspended in 500 ml water, and dissolved by titrating with 2 N KOH until its pH stabilize at 6.5. The rest of the ingredients are added and dissolved. Finally total volume is adjusted to 1 L.
   Acetic acid17ml
   Propionic acid6ml
   Butyric acid4ml
   iso-Butyric acid1ml
   n-Valeric acid1ml
   iso-Valeric acid1ml
   DL-α-Methylbutyric acid1ml
  ***Vitamin mixture
   Folic acid0.2mg
   p-Aminobenzoic acid20mg
   Choline chloride100mg
   Distilled water1L