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Medium Detail Information

Medium No.
Methanobacterium Medium
 Bacto Yeast Extract (Difco)0.2g
 Sodium acetate0.8g
 Vitamin solution*10ml
 Trace element solution**10ml
 Distilled water1L
  Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a H2/CO2 atmosphere (80/20). Concentrated cysteine-HCl and sodium sulfide solutions are sterilized separately under a N2 atmosphere in tightly closed vessels. Prior to inoculation, add the filter-sterile vitamin solution, cysteine-HCl and Na2S·9H2O. Pressurize the inoculated vessels to 150 kPa with H2/CO2 (80/20).
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.