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Medium Detail Information

Medium No.
1068 
Medium
Medium for strains JA145, JA193, JA310, JA334, JA415, JA430, JA447 and JA643 
Composition
 KH2PO40.5g
 MgSO4·7H2O0.2g
 NaCl0.4g
 NH4Cl0.6g
 CaCl2·2H2O0.05g
 Sodium pyruvate3g
 Yeast extract0.5g
 Ferric citrate solution* (0.1%)5ml
 Vitamin B12 (0.002% (w/v))1ml
 Trace element solution SL7**1ml
 Distilled water1L
 pH 7 
  Mix ingredients except for vitamin B12 and autoclave under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add vitamin B12 solution sterilized by filtration.
  For strain JA310, add 2 mM Na2S2O3 (separately sterilized).
       
  *Soluble in hot water.
       
  **Trace element solution SL7
   HCl (25%; 7.7 M)10ml
   FeCl2·4H2O1.5g
   ZnCl270mg
   MnCl2·4H2O100mg
   H3BO360mg
   CoCl2·6H2O200mg
   CuCl2·2H2O20mg
   NiCl2·6H2O20mg
   Na2MoO4·2H2O40mg
   Distilled water990ml
  Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
 
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