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Medium Detail Information

Medium No.	1068
Medium	Medium for strains JA145, JA193, JA310, JA334, JA415, JA430, JA447 and JA643
Composition
KH2PO4 0.5 g   MgSO4·7H2O 0.2 g   NaCl 0.4 g   NH4Cl 0.6 g   CaCl2·2H2O 0.05 g   Sodium pyruvate 3 g   Yeast extract 0.5 g   Ferric citrate solution* (0.1%) 5 ml   Vitamin B12 (0.002% (w/v)) 1 ml   Trace element solution SL7** 1 ml   Distilled water 1 L   pH 7       Mix ingredients except for vitamin B12 and autoclave under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add vitamin B12 solution sterilized by filtration.     For strain JA310, add 2 mM Na2S2O3 (separately sterilized).                   *Soluble in hot water.                   **Trace element solution SL7       HCl (25%; 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 60 mg       CoCl2·6H2O 200 mg       CuCl2·2H2O 20 mg       NiCl2·6H2O 20 mg       Na2MoO4·2H2O 40 mg       Distilled water 990 ml     Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
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