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Medium Detail Information

Medium No.
SYPG Medium
 Sea salts (SIGMA)35g
 Wolfe's mineral elixier*1ml
 Yeast extract0.5g
 Trypticase Peptone (BBL)0.25g
 Sodium glutamate0.1g
 Vitamin solution**10ml
 Distilled water1L
 pH 7.4-7.8 
  Dissolve ingredients except for carbonate and vitamins and sparge solution with N2/CO2 (80/20) gas mixture until it is anoxic and saturated with CO2 (at least 30 min.). Dispense solution in vials suitable for anaerobic cultures to 50% of volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Add vitamins from a sterile anoxic stock solution prepared under N2 and carbonate form an autoclaved stock solution prepared under N2/CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust the final pH of the medium to 7.4-7.8 using a sterile solution of sodium carbonate (5% w/v).
  *Wolfe's mineral elixier
   Distilled water1L
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L