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Medium Detail Information

Medium No.
1070
Medium
SYPG Medium
Composition
 Sea salts (SIGMA)35g
 Wolfe's mineral elixier*1ml
 NH4Cl0.1g
 KH2PO40.05g
 Yeast extract0.5g
 Trypticase Peptone (BBL)0.25g
 Sodium glutamate0.1g
 Na2CO30.5g
 Vitamin solution**10ml
 Distilled water1L
 pH 7.4-7.8 
  Dissolve ingredients except for carbonate and vitamins and sparge solution with N2/CO2 (80/20) gas mixture until it is anoxic and saturated with CO2 (at least 30 min.). Dispense solution in vials suitable for anaerobic cultures to 50% of volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Add vitamins from a sterile anoxic stock solution prepared under N2 and carbonate form an autoclaved stock solution prepared under N2/CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust the final pH of the medium to 7.4-7.8 using a sterile solution of sodium carbonate (5% w/v).
       
  *Wolfe's mineral elixier
   MgSO4·7H2O30g
   MnSO4·H2O5g
   NaCl10g
   FeSO4·7H2O1g
   CoCl2·6H2O1.8g
   CaCl2·2H2O1g
   ZnSO4·7H2O1.8g
   CuSO4·5H2O0.1g
   KAl(SO4)2·12H2O0.18g
   H3BO30.1g
   Na2MoO4·2H2O0.1g
   (NH4)2Ni(SO4)2·6H2O2.8g
   Na2WO4·2H2O0.1g
   Na2SeO40.1g
   Distilled water1L
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
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