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Medium Detail Information

Medium No.
1a Medium
 Trace metal solution*1ml
 Distilled water1L
 pH 7 
  Dispense the medium into suitable culture vessels, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. After cooling to room temperature, the pH is adjusted to pH 7 by adding of sterile pH-adjusting agent with a syringe without opening the vessel. Prior to inoculation, aseptically add the filter-sterilized methane gas to the vessel. The methane and air ratio in the headspace is about 1:4. Methane should be of high purity.
  Trace metal solution*
   Distilled water1L