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Medium Detail Information

Medium No.
1a Medium
  NaNO3 1 g
  MgSO4·7H2O 0.1 g
  Na2HPO4·2H2O 0.5 g
  KH2PO4 0.22 g
  CaCl2·6H2O 0.03 g
  FeSO4·7H2O 2 mg
  Trace metal solution* 1 ml
  Distilled water 1 L
  pH 7  
    Dispense the medium into suitable culture vessels, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. After cooling to room temperature, the pH is adjusted to pH 7 by adding of sterile pH-adjusting agent with a syringe without opening the vessel. Prior to inoculation, aseptically add the filter-sterilized methane gas to the vessel. The methane and air ratio in the headspace is about 1:4. Methane should be of high purity.
    *Trace metal solution
      ZnSO4·7H2O 0.44 g
      CuSO4·5H2O 0.2 g
      MnSO4·2H2O 0.17 g
      Na2MoO4·2H2O 0.06 g
      H3BO3 0.1 g
      CoCl2·6H2O 0.08 g
      Distilled water 1 L