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Medium Detail Information

Medium No.	1107
Medium	Medium for strains JA430
Composition
KH2PO4 0.5 g   MgSO4·7H2O 3 g   NaCl 50 g   NH4Cl 0.68 g   CaCl2·2H2O 0.15 g   Sodium pyruvate 3 g   Yeast extract 0.5 g   Ferric citrate solution* (0.1%) 5 ml   NaHCO3 (10% (w/v)) 15 ml   Na2S·9H2O 0.24 g   Vitamin B12 (0.002% (w/v)) 1 ml   Trace element solution SL7* 1 ml   Resazurin 1 mg   Distilled water 1 L   pH7.5       Mix ingredients except for vitamin B12, NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin B12 and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them.                   *Soluble in hot water.                   **Trace element solution SL7       HCl (25%; 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 60 mg       CoCl2·6H2O 200 mg       CuCl2·2H2O 20 mg       NiCl2·6H2O 20 mg       Na2MoO4·2H2O 40 mg       Distilled water 990 ml     Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
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