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Medium Detail Information

Medium No.
Marine Methanosaeta Medium
 KP buffer*10ml
 Sodium acetate6.56g
 Bacto Yeast Extract (Difco)1.5g
 Bacto Tryptone (Difco)0.4g
 Trace element solution**2ml
 Vitamin solution***10ml
 Coenzyme M0.14g
 Distilled water1L
  Mix ingredients except for KP buffer, vitamin solution and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. Separately autoclave KP buffer and Na2S·9H2O (5% solution) under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically and anaerobically add KP buffer, vitamin, and Na2S·9H2O solutions.
  *KP buffer
   Distilled water1L
  Autoclaved under N2.
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
  ***Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L