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Medium Detail Information

Medium No.
Nitrate mineral salts medium (NMS) + 0.01% Tryptone
 Chelated Iron Solution*2ml
 Trace Element Solution**0.5ml
 Bacto Tryptone (Difco)0.1g
 Purified Agar (e.g., Oxoid L28)12.5g
 Distilled deionized water1L
 pH 6.8 
  Dispense the medium into suitable culture vessels, seal with butyl rubber stoppers and autoclave at 121°C for 15 min. Prior to inoculation, aseptically add the filter-sterilized methane gas to the vessel. The methane and air ratio in the headspace is about 1:4. Methane should be of high purity.
  *Chelated Iron Solution
   Ferric ammonium citrate***0.1g
   EDTA, sodium salt0.2g
   HCl (concentrated)0.3ml
   Distilled water100ml
  **Trace Element Solution
   Distilled water1L
  Autoclave at 121C for 15 minutes.
  ***Ferric ammonium citrate
  0.05 g of Ferric chloride may be substituted.