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Medium Detail Information

Medium No.
1116
Medium
Nitrate mineral salts medium (NMS) + 0.01% Tryptone
Composition
 MgSO4·7H2O1g
 CaCl2·6H2O0.2g
 Chelated Iron Solution*2ml
 KNO31g
 Trace Element Solution**0.5ml
 KH2PO40.272g
 Na2HPO4·12H2O0.717g
 Bacto Tryptone (Difco)0.1g
 Purified Agar (e.g., Oxoid L28)12.5g
 Distilled deionized water1L
 pH 6.8 
  Dispense the medium into suitable culture vessels, seal with butyl rubber stoppers and autoclave at 121°C for 15 min. Prior to inoculation, aseptically add the filter-sterilized methane gas to the vessel. The methane and air ratio in the headspace is about 1:4. Methane should be of high purity.
       
  *Chelated Iron Solution
   Ferric ammonium citrate***0.1g
   EDTA, sodium salt0.2g
   HCl (concentrated)0.3ml
   Distilled water100ml
       
  **Trace Element Solution
   EDTA500mg
   FeSO4·7H2O200mg
   ZnSO4·7H2O10mg
   MnCl2·4H2O3mg
   H3BO330mg
   CoCl2·6H2O20mg
   CaCl2·2H2O1mg
   NiCl2·6H2O2mg
   Na2MoO4·2H2O3mg
   Distilled water1L
  Autoclave at 121C for 15 minutes.
       
  ***Ferric ammonium citrate
  0.05 g of Ferric chloride may be substituted.
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