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Medium Detail Information

Medium No.	1116
Medium	Nitrate mineral salts medium (NMS) + 0.01% Tryptone
Composition
MgSO4·7H2O 1 g   CaCl2·6H2O 0.2 g   Chelated Iron Solution* 2 ml   KNO3 1 g   Trace Element Solution** 0.5 ml   KH2PO4 0.272 g   Na2HPO4·12H2O 0.717 g   Bacto Tryptone (Difco) 0.1 g   Purified Agar (e.g., Oxoid L28) 12.5 g   Distilled deionized water 1 L   pH 6.8       Dispense the medium into suitable culture vessels, seal with butyl rubber stoppers and autoclave at 121°C for 15 min. Prior to inoculation, aseptically add the filter-sterilized methane gas to the vessel. The methane and air ratio in the headspace is about 1:4. Methane should be of high purity.                   *Chelated Iron Solution       Ferric ammonium citrate*** 0.1 g       EDTA, sodium salt 0.2 g       HCl (concentrated) 0.3 ml       Distilled water 100 ml                   **Trace Element Solution       EDTA 500 mg       FeSO4·7H2O 200 mg       ZnSO4·7H2O 10 mg       MnCl2·4H2O 3 mg       H3BO3 30 mg       CoCl2·6H2O 20 mg       CaCl2·2H2O 1 mg       NiCl2·6H2O 2 mg       Na2MoO4·2H2O 3 mg       Distilled water 1 L     Autoclave at 121C for 15 minutes.                   ***Ferric ammonium citrate     0.05 g of Ferric chloride may be substituted.
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