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Medium Detail Information

Medium No.	1128
Medium	Acidianus medium
Composition
(NH4)2SO4 1.3 g   KH2PO4 0.28 g   MgSO4·7H2O 0.25 g   CaCl2·2H2O 0.07 g   FeCl3·6H2O 0.02 g   MnCl2·4H2O 1.8 mg   Na2B4O7·10H2O 4.5 mg   ZnSO4·7H2O 0.22 mg   CuCl2·2H2O 0.05 mg   Na2MoO4·2H2O 0.03 mg   VOSO4·xH2O 0.03 mg   CoSO4·7H2O 0.01 mg   Bacto Yeast Extract (Difco) 0.5 g   Sulfur (powder) 1 g   Resazurin 1 mg   Na2S·9H2O 0.5 g   Distilled water 1 L     Mix ingredients except for Bacto Yeast Extract, sulfur and Na2S·9H2O, and adjust pH to 2.0 with H2SO4. Dispense the medium into suitable culture vessels under a stream of H2/CO2 (80/20) and seal with butyl rubber stoppers. Separately autoclave Bacto Yeast Extract and Na2S·9H2O as 5% solutions under a N2 atmosphere. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of successive days. Prior to inoculation, add Bacto Yeast Extract, sulfur and Na2S·9H2O, and pressurize the inoculated vessels to 150 kPa with H2/CO2.
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