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Medium Detail Information

Medium No.
1128
Medium
Acidianus medium
Composition
 (NH4)2SO41.3g
 KH2PO40.28g
 MgSO4·7H2O0.25g
 CaCl2·2H2O0.07g
 FeCl3·6H2O0.02g
 MnCl2·4H2O1.8mg
 Na2B4O7·10H2O4.5mg
 ZnSO4·7H2O0.22mg
 CuCl2·2H2O0.05mg
 Na2MoO4·2H2O0.03mg
 VOSO4·xH2O0.03mg
 CoSO4·7H2O0.01mg
 Bacto Yeast Extract (Difco)0.5g
 Sulfur (powder)1g
 Resazurin1mg
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except for Bacto Yeast Extract, sulfur and Na2S·9H2O, and adjust pH to 2.0 with H2SO4. Dispense the medium into suitable culture vessels under a stream of H2/CO2 (80/20) and seal with butyl rubber stoppers. Separately autoclave Bacto Yeast Extract and Na2S·9H2O as 5% solutions under a N2 atmosphere. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of successive days. Prior to inoculation, add Bacto Yeast Extract, sulfur and Na2S·9H2O, and pressurize the inoculated vessels to 150 kPa with H2/CO2.
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