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Medium Detail Information

Medium No.
1150
Medium
Thermotoga hypogea medium
Composition
 K2HPO40.3g
 KH2PO40.3g
 NH4Cl1g
 MgCl2·6H2O0.2g
 CaCl2·2H2O0.1g
 KCl0.1g
 Sodium acetate0.5g
 NaCl10g
 Na2S2O3·5H2O5g
 Bacto Yeast Extract (Difco)2g
 Polypeptone2g
 Xylose3g
 NaHCO32g
 Trace element solution*10ml
 Resazurin1mg
 Cysteine-HCl0.5g
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except for xylose, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave xylose (as 15% solution) cysteine-HCl (as 5% solution) and Na2S·9H2O solutions (as 5% solution) under a N2 atmosphere. Prior to inoculation, add the xylose, cysteine-HCl, and Na2S·9H2O solutions. Final pH of the complete medium should be 7.2-7.4.
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   KAl(SO4)2·12H2O0.018g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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