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Medium No.
Thermotoga hypogea medium
 Sodium acetate0.5g
 Bacto Yeast Extract (Difco)2g
 Trace element solution*10ml
 Distilled water1L
  Mix ingredients except for xylose, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave xylose (as 15% solution) cysteine-HCl (as 5% solution) and Na2S·9H2O solutions (as 5% solution) under a N2 atmosphere. Prior to inoculation, add the xylose, cysteine-HCl, and Na2S·9H2O solutions. Final pH of the complete medium should be 7.2-7.4.
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.