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Medium Detail Information

Medium No.
Medium for aerobic growth of strain sk43H
 Sodium acetate0.82g
 Trace element mixture*1ml
 Selenite-tungstate solution**1ml
 Vitamin solution***1ml
 Thiamine solution****1ml
 Vitamin B12 solution*****1ml
 Thiosulfate solution******1.5ml
 Distilled water1L
 pH 6.8 
  Mix ingredients except for vitamin, thiamine, cyanocobalamin and thiosulfate solutions. Dispense the medium into suitable culture vessels and autoclave at 121°C for 15 min. After autoclaved, aseptically add other ingredients
  *Trace element mixture
   HCl (25%; 7.7 M)12.5ml
   Distilled water987ml
  Sterilized by autoclaving.
  **Selenite-tungstate solution
   Distilled water1L
  Sterilized by autoclaving.
  ***Vitamine solution
   p-Aminobenzoic acid4mg
   Nicotinic acid10mg
   Sodium phosphate buffer (10 mM; pH7.1)100ml
  Sterilized by filtration.
  ****Thiamine solution
   Sodium phosphate buffer (25 mM; pH 3.4)100ml
  Sterilized by filtration.
  *****Vitamin B12 solution
   Distilled water100ml
  Sterilized by filtration.
  ******Thiosulfate solution (1.0 M)
   Distilled water100ml
  Sterilized by filtration and stored under N2 gas.