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Medium Detail Information

Medium No.
1163
Medium
Medium for Strain Ug1
Composition
 Na2SiO30.03g
 NH4Cl0.5g
 KH2PO40.5g
 CaCl2·H2O0.14g
 KCl0.3g
 MgCl2·6H2O0.2g
 NaHCO30.1g
 Yeast extract0.1g
 Trace element solution SL-6*1ml
 Vitamin solution**10ml
 Resazurin0.5mg
 Sodium pyruvate2g
 Na2S·9H2O (neutralized)***0.15g
 Distilled water1L
 pH 6.2 
  Mix ingredients except for vitamin, bicarbonate and sulfide. Dispense the medium into suitable culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add filter-sterilized vitamin and bicarbonate solutions and adjust pH to 6.2 using compact pH meter. Prior to inoculation, aseptically add the sterilized sulfide solution.
       
  *Trace element solution SL-6
   ZnSO4·7H2O0.1g
   MnCl2·4H2O0.03g
   H3BO30.3g
   CoCl2·6H2O0.2g
   CuCl2·2H2O0.01g
   NiCl2·6H2O0.02g
   Na2MoO4·2H2O0.03g
   Distilled water1L
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
       
  ***Na2S·9H2O (neutralized)
   Na2S·9H2O5g
   Distilled water100ml
  The sulfide solution is prepared in a vessel sealed with a butyl rubber stopper. Autoclave at 121℃ for 15 min under a N2 atmosphere. After cooling to room temperature, the pH is adjusted to about 7.3 by adding of sterile 2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow colour indicates the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.
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