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Medium Detail Information

Medium No.
Medium for Strain Ug1
 Yeast extract0.1g
 Trace element solution SL-6*1ml
 Vitamin solution**10ml
 Sodium pyruvate2g
 Na2S·9H2O (neutralized)***0.15g
 Distilled water1L
 pH 6.2 
  Mix ingredients except for vitamin, bicarbonate and sulfide. Dispense the medium into suitable culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add filter-sterilized vitamin and bicarbonate solutions and adjust pH to 6.2 using compact pH meter. Prior to inoculation, aseptically add the sterilized sulfide solution.
  *Trace element solution SL-6
   Distilled water1L
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
  ***Na2S·9H2O (neutralized)
   Distilled water100ml
  The sulfide solution is prepared in a vessel sealed with a butyl rubber stopper. Autoclave at 121℃ for 15 min under a N2 atmosphere. After cooling to room temperature, the pH is adjusted to about 7.3 by adding of sterile 2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow colour indicates the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.