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Medium Detail Information

Medium No.
GYS Medium
  Solution A* 500 ml
  Solution B** 500 ml
    Agar solid medium of GYS contained 2% agar. In order to prevent the hydrolysis of agar and glucose by autoclaving at low pH condition, Solutions A and B are autoclaved separately and combined before solidified (50-60℃).
    *Solution A
      KH2PO4 1 g
      (NH4)2SO4 1 g
      Yeast extract 0.3 g
      MgCl2·6H2O 0.2 g
      CaCl2 45 mg
      NaCl 0.2 g
      Trace element*** 1 ml
      Distilled water 500 ml
    Adjust pH to 3.5-5.0 with 1 N H2SO4 for each strain (see Comments in catalogue) .
    **Solution B
      Glucose 2 g
      Agar (if needed) 15 g
      Distilled water 500 ml
    ***Trace element solution
      2Na-EDTA 5 g
      FeCl2·4H2O 1 g
      H3BO3 0.3 g
      CoCl2·6H2O 0.11 g
      ZnCl2 50 mg
      MnCl2·4H2O 30 mg
      Na2MoO4·2H2O 30 mg
      NiSO4·6H2O 30 mg
      CuCl2·2H2O 10 mg
      Distilled water 1 L