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Medium Detail Information

Medium No.
Methanohalobium medium
 Coenzyme M0.142g
 Bacto Yeast Extract (Difco)0.5g
 Vitamin solution*10ml
 Trace elements solution**1ml
 Distilled water1L
  Mix ingredients except for vitamin solution, trimethylamine-HCl, NaHCO3 and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave Na2S·9H2O solution as 5% solution under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, 50% trimethylamine-HCl and 5% NaHCO3 solutions, and Na2S·9H2O. Adjust pH of the medium to 7.4 with filter-sterile 5% Na2CO3 solution (approximately final concentration of Na2CO3 is 0.5 g/L).
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.