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Medium Detail Information

Medium No.
1172
Medium
Methanosalsum medium
Composition
 NaCl40g
 NH4Cl1g
 MgCl2·6H2O3.5g
 MgSO4·7H2O3g
 KCl1g
 K2HPO40.4g
 Vitamin solution*10ml
 Trace elements solution**10ml
 Bacto Yeast Extract (Difco)2g
 Polypeptone2g
 Trimethylamine-HCl2g
 Resazurin1mg
 Carbonate solution***50ml
 Cysteine-HCl0.5g
 Na2S·9H2O0.25g
 Distilled water950ml
  Mix ingredients except for vitamin, carbon solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 5% solutions under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, filter-sterile carbonate solution, cysteine-HCl and Na2S·9H2O. Final pH is 8.5-9.5.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   KAl(SO4)2·12H2O0.02g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  ***Carbonate solution
   NaHCO360g
   Na2CO340g
   Distilled water1L
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