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Medium Detail Information

Medium No.
Methanosalsum medium
 Vitamin solution*10ml
 Trace elements solution**10ml
 Bacto Yeast Extract (Difco)2g
 Carbonate solution***50ml
 Distilled water950ml
  Mix ingredients except for vitamin, carbon solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 5% solutions under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, filter-sterile carbonate solution, cysteine-HCl and Na2S·9H2O. Final pH is 8.5-9.5.
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
  ***Carbonate solution
   Distilled water1L