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Medium Detail Information

Medium No.
1173
Medium
Methanomethylovorans medium
Composition
 KH2PO40.2g
 NH4Cl0.25g
 NaCl1g
 MgCl2·6H2O0.4g
 KCl0.5g
 CaCl2·2H2O0.15g
 Vitamin solution*10ml
 Trace elements solution**10ml
 FeSO4·7H2O0.02g
 Trimethylamine-HCl2g
 Methanol0.8ml
 Resazurin1mg
 NaHCO32.5g
 Na2S·9H2O0.3g
 Distilled water1L
  Mix ingredients except for vitamin, NaHCO3, FeSO4·7H2O, methanol and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave FeSO4·7H2O and Na2S·9H2O solutions as 0.2% and 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, filter-sterile 5% NaHCO3 solution, filter-sterile methanol, FeSO4·7H2O and Na2S·9H2O. Adjust final pH to 7.2-7.4 if necessary..
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   KAl(SO4)2·12H2O0.02g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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