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Medium Detail Information

Medium No.	1173
Medium	Methanomethylovorans medium
Composition
KH2PO4 0.2 g   NH4Cl 0.25 g   NaCl 1 g   MgCl2·6H2O 0.4 g   KCl 0.5 g   CaCl2·2H2O 0.15 g   Vitamin solution* 10 ml   Trace elements solution** 10 ml   FeSO4·7H2O 0.02 g   Trimethylamine-HCl 2 g   Methanol 0.8 ml   Resazurin 1 mg   NaHCO3 2.5 g   Na2S·9H2O 0.3 g   Distilled water 1 L     Mix ingredients except for vitamin, NaHCO3, FeSO4·7H2O, methanol and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave FeSO4·7H2O and Na2S·9H2O solutions as 0.2% and 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, filter-sterile 5% NaHCO3 solution, filter-sterile methanol, FeSO4·7H2O and Na2S·9H2O. Adjust final pH to 7.2-7.4 if necessary..                   *Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 0.01 mg       Distilled water 1 L                   **Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       KAl(SO4)2·12H2O 0.02 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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