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Medium Detail Information

Medium No.
1174
Medium
Methanomethylovorans thermophila medium
Composition
 Na2HPO4·2H2O0.53g
 KH2PO40.41g
 NH4Cl0.3g
 CaCl2·2H2O0.11g
 MgCl2·6H2O0.1g
 NaCl0.3g
 Vitamin solution*10ml
 Trace elements solution**10ml
 Bacto Yeast Extract (Difco)0.2g
 Methanol2ml
 Resazurin0.5mg
 NaHCO34g
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except for vitamin, CaCl2·2H2O, MgCl2·6H2O, methanol and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave Na2S·9H2O solution as 5% solution under a N2 atmosphere. Separately autoclave 1.1% CaCl2·2H2O and 1% MgCl2·6H2O solution under a N2/CO2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, mgnesium and calcium solution, filter-sterile methanol and Na2S·9H2O. Final pH is 7.0-7.2.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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