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Medium Detail Information

Medium No.
Methanomethylovorans thermophila medium
 Vitamin solution*10ml
 Trace elements solution**10ml
 Bacto Yeast Extract (Difco)0.2g
 Distilled water1L
  Mix ingredients except for vitamin, CaCl2·2H2O, MgCl2·6H2O, methanol and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave Na2S·9H2O solution as 5% solution under a N2 atmosphere. Separately autoclave 1.1% CaCl2·2H2O and 1% MgCl2·6H2O solution under a N2/CO2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, mgnesium and calcium solution, filter-sterile methanol and Na2S·9H2O. Final pH is 7.0-7.2.
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.