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Medium Detail Information

Medium No.	1198
Medium	DG18: Dichloran 18% Glycerol Agar
Composition
Peptone 5 g   Glucose 10 g   KH2PO4 1 g   MgSO4·7H2O 0.5 g   Dichloran (0.2% in ethanol) 1.0 ml   Glycerol 220 g   Chloramphenicol 0.1 g   Agar 15.0 g   Distilled water 1 L     Add minor ingredients and agar to ca. 800 ml distilled water. Steam to dissolve agar, then make to one litre with distilled water. Add 220 g glycerol and sterilize by autoclaving at 121°C for 15 min. The final aw is 0.955, final pH 5.6±0.2.
Comment	Commercial Dichloran glycerol agar base (Oxoid, MERCK, etc.) is also available.