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Medium No.
1198
Medium
DG18: Dichloran 18% Glycerol Agar
Composition
 Peptone5g
 Glucose10g
 KH2PO41g
 MgSO4·7H2O0.5g
 Dichloran (0.2% in ethanol)1.0ml
 Glycerol220g
 Chloramphenicol0.1g
 Agar15.0g
 Distilled water1L
  Add minor ingredients and agar to ca. 800 ml distilled water. Steam to dissolve agar, then make to one litre with distilled water. Add 220 g glycerol and sterilize by autoclaving at 121°C for 15 min. The final aw is 0.955, final pH 5.6±0.2.
Comment
Commercial Dichloran glycerol agar base (Oxoid, MERCK, etc.) is also available.