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Medium Detail Information

Medium No.
Medium for Strain GH1
 Bacto Yeast Extract (Difco)5g
 Tween 800.025g
 Salts solution**5ml
 Vitamin solution***10ml
 Trace element solution SL-10****1ml
 Distilled water1L
 pH 6.0 
  Mix ingredients except for vitamins and sulfide, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically and anaerobically add them.
  *Wako Pure Chemical Ind., Ltd., Osaka, Japan.
  **Salts solution
   Distilled water1L
  ***Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
  ****Trace element solution SL-10
   HCl (25%; 7.7 M)10ml
   Distilled water990ml