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Medium Detail Information

Medium No.
1217
Medium
Medium for Strain GH1
Composition
 Bacto Yeast Extract (Difco)5g
 Hipolypepton*5g
 Tween 800.025g
 Salts solution**5ml
 Vitamin solution***10ml
 Trace element solution SL-10****1ml
 Na2S·9H2O0.5g
 Resazurin1mg
 Distilled water1L
 pH 6.0 
  Mix ingredients except for vitamins and sulfide, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically and anaerobically add them.
       
  *Wako Pure Chemical Ind., Ltd., Osaka, Japan.
       
  **Salts solution
   MgSO4·7H2O40g
   MnSO4·4H2O2g
   FeSO4·7H2O2g
   NaCl2g
   Distilled water1L
       
  ***Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
       
  ****Trace element solution SL-10
   HCl (25%; 7.7 M)10ml
   FeCl2·4H2O1.5g
   ZnCl27mg
   MnCl2·4H2O100mg
   H3BO36mg
   CoCl2·6H2O190mg
   CuCl2·2H2O2mg
   NiCl2·6H2O24mg
   Na2MoO4·2H2O36mg
   Distilled water990ml
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