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Medium Detail Information

Medium No.
Medium for Strain JA746
 Sodium pyruvate2g
 Sodium succinate2g
 Sodium acetate2g
 Yeast extract0.4g
 Ferric citrate solution* (0.1%)5ml
 Trace element solution SL7**1ml
 NaHCO3 solution (10% w/v)15ml
 Vitamin B12 (0.002% (w/v))1ml
 Distilled water1L
 pH 7 
  Mix ingredients except vitamin B12, bicarbonate and sulfide, and dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically and anaerobically add vitamin B12 solution, bicarbonate solution and sulfide solution using sterile syringes. The bicarbonate and vitamin solutions should be sterilized by filtration. Adjust the pH of the medium to pH 7.
  *Soluble in hot water.
  **Trace element solution SL7
   HCl (25%; 7.7 M)10ml
   Distilled water990ml
  Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.