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Medium Detail Information

Medium No.
1226
Medium
Medium for Strain GK12
Composition
 NH4Cl1g
 KH2PO40.27g
 MgSO4·7H2O0.25g
 CaCl2·2H2O0.15g
 NaHCO32.5g
 Glucose10g
 Yeast extract3g
 Na2S·9H2O30g
 Cysteine-HCl30g
 Resazurin1mg
 Trace element solution*1ml
 Vitamin solution**1ml
 Distilled water1L
 pH unajusted 
  Mix ingredients except vitamin, bicarbonate, cysteine and sulfide, and dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically and anaerobically add vitamin solution, bicarbonate solution, and cysteine and sulfide solutions using sterile syringes. The bicarbonate and vitamin solutions should be sterilized by filtration.
       
  *Trace element solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O24mg
   AlCl313.3mg
   ZnCl20.1g
   CuCl2·2H2O25mg
   H3BO310mg
   Na2MoO4·2H2O24mg
   Na2WO4·2H2O3mg
   NiCl2·6H2O0.12g
   Na2SeO326mg
   Distilled water1L
  First dissolve NTA in 200 ml of water and adjust the pH to 6.5 with KOH.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B125mg
   Lipoic acid5mg
   Distilled water1L
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