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Medium Detail Information

Medium No.	1226
Medium	Medium for Strain GK12
Composition
NH4Cl 1 g   KH2PO4 0.27 g   MgSO4·7H2O 0.25 g   CaCl2·2H2O 0.15 g   NaHCO3 2.5 g   Glucose 10 g   Yeast extract 3 g   Na2S·9H2O 30 g   Cysteine-HCl 30 g   Resazurin 1 mg   Trace element solution* 1 ml   Vitamin solution** 1 ml   Distilled water 1 L   pH unajusted       Mix ingredients except vitamin, bicarbonate, cysteine and sulfide, and dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically and anaerobically add vitamin solution, bicarbonate solution, and cysteine and sulfide solutions using sterile syringes. The bicarbonate and vitamin solutions should be sterilized by filtration.                   *Trace element solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 24 mg       AlCl3 13.3 mg       ZnCl2 0.1 g       CuCl2·2H2O 25 mg       H3BO3 10 mg       Na2MoO4·2H2O 24 mg       Na2WO4·2H2O 3 mg       NiCl2·6H2O 0.12 g       Na2SeO3 26 mg       Distilled water 1 L     First dissolve NTA in 200 ml of water and adjust the pH to 6.5 with KOH.                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 5 mg       Lipoic acid 5 mg       Distilled water 1 L
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