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Medium Detail Information

Medium No.
Medium for Strain SR45
 Yeast extract1g
 Sodium acetate2.73g
 Trace element mixture*1ml
 Selenite-tungstate solution**1ml
 Bicarbonate solution***30ml
 Sulfide solution****1.5ml
 Distilled water970ml
 pH 6.8-7.0 
  Mix ingredients except for bicarbonate and sulfide solutions. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add other ingredients using sterile syringes, and the N2/CO2 (80/20) mixed gas is enclosed with the vessels. Adjust pH to 6.8-7.0 using compact pH meter.
  *Trace element mixture
   HCl (25%; 7.7 M)12.5ml
   Distilled water987ml
  Sterilized by autoclaving.
  **Selenite-tungstate solution
   Distilled water1L
  Sterilized by autoclaving.
  ***Bicarbonate solution (1.0 M)
   Distilled water100ml
  Saturated with CO2 gas. Tightly sealed in bottles filled with CO2 gas and then sterilized by autoclaving.
  ****Sulfide solution (1.0 M)
   Distilled water50ml
  Sterilized by filtration and stored under N2 gas.