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Medium Detail Information

Medium No.	1269
Medium	Medium for Strain T-1-35
Composition
KH2PO4 0.2 g   NH4Cl 0.25 g   NaCl 1 g   MgCl2·6H2O 0.4 g   KCl 0.5 g   CaCl2·2H2O 0.15 g   Cellobiose 10 g   Yeast Extract 1 g   Resazurin 2 mg   Na2S·9H2O 0.36 g   NaHCO3 2.52 g   Trace element SL-10* 1 ml   Selenite-tungstate solution** 1 ml   Vitamin solution*** 2 ml   Vitamin B12 solution**** 1 ml   Distilled water 1 L   pH 8 - 8.5       Mix ingredients except for vitamins, NaHCO3 and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamins and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them.                   *Trace element solution SL-10       HCl (25%; 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 6 mg       CoCl2·6H2O 190 mg       CuCl2·2H2O 2 mg       NiCl2·6H2O 24 mg       Na2MoO4·2H2O 36 mg       Distilled water 990 ml                   **Selenite-tungstate solution       NaOH 0.5 g       Na2SeO3·5H2O 3 mg       Na2WO4·2H2O 4 mg       Distilled water 1 L     Sterilized by autoclaving.                   ***Vitamine solution       p-Aminobenzoic acid 4 mg       Biotin 1 mg       Thiamine-HCl 10 mg       Distilled water 100 ml     Sterilized by filtration.                   ****Vitamin B12 solution       Cyanocobalamin 5 mg       Distilled water 100 ml     Sterilized by filtration.
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