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Medium Detail Information

Medium No.
Medium for Strain T-1-35
 Yeast Extract1g
 Trace element SL-10*1ml
 Selenite-tungstate solution**1ml
 Vitamin solution***2ml
 Vitamin B12 solution****1ml
 Distilled water1L
 pH 8 - 8.5 
  Mix ingredients except for vitamins, NaHCO3 and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamins and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them.
  *Trace element solution SL-10
   HCl (25%; 7.7 M)10ml
   Distilled water990ml
  **Selenite-tungstate solution
   Distilled water1L
  Sterilized by autoclaving.
  ***Vitamine solution
   p-Aminobenzoic acid4mg
   Distilled water100ml
  Sterilized by filtration.
  ****Vitamin B12 solution
   Distilled water100ml
  Sterilized by filtration.