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Medium Detail Information

Medium No.
1269
Medium
Medium for Strain T-1-35
Composition
 KH2PO40.2g
 NH4Cl0.25g
 NaCl1g
 MgCl2·6H2O0.4g
 KCl0.5g
 CaCl2·2H2O0.15g
 Cellobiose10g
 Yeast Extract1g
 Resazurin2mg
 Na2S·9H2O0.36g
 NaHCO32.52g
 Trace element SL-10*1ml
 Selenite-tungstate solution**1ml
 Vitamin solution***2ml
 Vitamin B12 solution****1ml
 Distilled water1L
 pH 8 - 8.5 
  Mix ingredients except for vitamins, NaHCO3 and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamins and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them.
       
  *Trace element solution SL-10
   HCl (25%; 7.7 M)10ml
   FeCl2·4H2O1.5g
   ZnCl270mg
   MnCl2·4H2O100mg
   H3BO36mg
   CoCl2·6H2O190mg
   CuCl2·2H2O2mg
   NiCl2·6H2O24mg
   Na2MoO4·2H2O36mg
   Distilled water990ml
       
  **Selenite-tungstate solution
   NaOH0.5g
   Na2SeO3·5H2O3mg
   Na2WO4·2H2O4mg
   Distilled water1L
  Sterilized by autoclaving.
       
  ***Vitamine solution
   p-Aminobenzoic acid4mg
   Biotin1mg
   Thiamine-HCl10mg
   Distilled water100ml
  Sterilized by filtration.
       
  ****Vitamin B12 solution
   Cyanocobalamin5mg
   Distilled water100ml
  Sterilized by filtration.
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