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Medium Detail Information

Medium No.
1275
Medium
Modified Tc medium
Composition
 NaCl25g
 KCl0.33g
 NH4Cl0.25g
 MgCl2·6H2O2.8g
 MgSO4·7H2O3.4g
 FeSO4·7H2O0.025g
 K2HPO40.3g
 NaBr0.01g
 Trace elements solution*10ml
 Vitamin solution**10ml
 Bacto Yeast Extract (Difco)3g
 Bacto Tryptone (Difco)3g
 NaHCO32.5g
 Sulfur (powder)3g
 Resazurin1mg
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except vitamin solution, sulfur and Na2S·9H2O, and autoclave under a N2/CO2 (80/20) atmosphere. Filter-sterilize vitamin solution. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each 3 successive days. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add vitamin, sulfur and Na2S·9H2O. Final pH is 6.8.
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.8mg
   Na2WO40.8mg
   KAl(SO4)2·12H2O4mg
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH is 7.0.
       
  ** Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
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