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Medium Detail Information

Medium No.
Modified Tc medium
 Trace elements solution*10ml
 Vitamin solution**10ml
 Bacto Yeast Extract (Difco)3g
 Bacto Tryptone (Difco)3g
 Sulfur (powder)3g
 Distilled water1L
  Mix ingredients except vitamin solution, sulfur and Na2S·9H2O, and autoclave under a N2/CO2 (80/20) atmosphere. Filter-sterilize vitamin solution. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each 3 successive days. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add vitamin, sulfur and Na2S·9H2O. Final pH is 6.8.
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH is 7.0.
  ** Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L