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Medium Detail Information

Medium No.
1276
Medium
Methanomethylovorans medium
Composition
 NaCl1g
 KCl0.5g
 MgCl2·6H2O0.4g
 CaCl2·2H2O0.1g
 KH2PO40.2g
 NH4Cl0.5g
 Bacto Yeast Extract (Difco)0.2g
 Trimethylamine-HCl1.5g
 Trace elements solution*2ml
 Vitamin solution**2ml
 NaHCO31.7g
 Na2S·9H2O0.5g
 Resazurin1mg
 Distilled water1L
  Mix ingredients except trimethylamine, vitamin solution, NaHCO3 and Na2S·9H2O, and autoclave under a N2/CO2 (80/20) atmosphere. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile trimethylamine as 15% solution, NaHCO3 as 8% solution and vitamin solution to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution. Pressurize the inoculated vessels to 150 kPa with N2/CO2.
       
  *Trace elements solution
   Nitrilotriacetic acid4.5g
   FeCl2·4H2O0.4g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.17g
   ZnCl20.1g
   CaCl2·2H2O0.02g
   H3BO30.019g
   Na2MoO4·2H2O0.01g
   NiCl2·6H2O0.03g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
 Adjust pH to 7.0 with 1 N KOH 
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
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