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Medium Detail Information

Medium No.
Methanomethylovorans medium
 Bacto Yeast Extract (Difco)0.2g
 Trace elements solution*2ml
 Vitamin solution**2ml
 Distilled water1L
  Mix ingredients except trimethylamine, vitamin solution, NaHCO3 and Na2S·9H2O, and autoclave under a N2/CO2 (80/20) atmosphere. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile trimethylamine as 15% solution, NaHCO3 as 8% solution and vitamin solution to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution. Pressurize the inoculated vessels to 150 kPa with N2/CO2.
  *Trace elements solution
   Nitrilotriacetic acid4.5g
   Distilled water1L
 Adjust pH to 7.0 with 1 N KOH 
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L