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Medium Detail Information

Medium No.
 Ferric EDTA solution*0.1ml
 Molybdate solution**1ml
 Trace elements solution***2ml
 0.4 M Phosphate buffer ****10ml
 Artificial seawater1L
 pH 6.8 
  Mix ingredients except for phosphate buffer, adjust pH to 6.8, and dispense the medium into suitable culture vessels, seal with butyl rubber stoppers, and autoclave at 121°C for 20 min. After autoclaved, aseptically add phosphate buffer which is separately autoclaved. Prior to inoculation, aseptically add the filter-sterilized methane gas to the vessel. The methane and air ratio in the headspace is about 1:4. Methane should be of high purity.
  *Ferric EDTA solution
   Distilled water1L
  **Molybdate solution
   Distilled water1L
  ***Trace elements solution
   Distilled water1L
  Dissolve the above in the specified order in distilled water and dilute to 1 litre. Store in the dark.
  ****0.4 M Phosphate buffer:
  Mix 0.4 M Na2HPO4 and 0.4 M KH2PO4 solutions, and adjust pH to 6.8 (approximately volume rate is 2:1).