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Medium Detail Information

Medium No.
Modified 289 medium
 Sodium thioglycolate0.05g
 Sodium tartrate0.37g
 Sodium succinate0.37g
 Sodium lactate0.05g
 Vitamin mixture*5ml
 Ferric quinate solution**2ml
 HMSS solution***1ml
 Distilled water1L
 Adjust pH to 6.75 with 1 N NaOH 
  Good growth can be obtained in semi-solid medium (1 g of agar in 1 liter).
  The anaerobic growth with broth was carried out in an anaerobic jar equipped with a MicroAero gas pack (Mitsubishi Gas Chemical Company Inc., Tokyo, Japan).
  *Vitamin mixture
   Folic acid0.2mg
   p-Aminobenzoic acid20mg
   Choline chloride100mg
   Distilled water1L
  **Ferric quinate solution
   Quinic acid0.19g
   Distilled water1L
  ***HMSS solution
   Nitrilotriacetic acid10g
   Metal 44 solution****50ml
   Distilled water1L
  Neutralize the nitrilotriacetic acid with KOH. Dissolve the remaining ingredients and adjust the pH to 7.2 with KOH.
  ****Metal 44 solution
   Distilled water1L