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Medium Detail Information

Medium No.
Methanosarcina Medium
 KP buffer*10ml
 Bacto Yeast Extract (Difco)2g
 Methanol (10%)10ml
 Vitamin solution**10ml
 Trace element solution***10ml
 Distilled water1L
  Mix ingredients except for KP buffer, metanol, vitamin, cysteine-HCl and Na2S·9H2O, and autoclave under a N2 atmosphere. KP buffer, and concentrated cysteine-HCl and sodium sulfide solutions are sterilized separately under a N2 atmosphere in tightly closed vessels. Prior to inoculation, add the filter-sterile methanol and vitamin , KP buffer, cysteine-HCl and Na2S·9H2O.
  *KP buffer
   Distilled water1L
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  ***Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.