Search Results Detail

Medium Detail Information

Medium No.
Methanohalophilus Medium
 Vitamin solution*2ml
 Trace element solution**1ml
 Distilled water1L
  Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 5% solutions under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, add vitamin solution, cysteine-HCl and Na2S·9H2O.
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.