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Medium Detail Information

Medium No.
Medium for Strain KC3
  Hipolypepton* 0.5 g
  Yeast extract 0.1 g
  CaCO3 0.2 g
  NaCl 0.5 g
  MgSO4 ·7H2O 0.35 g
  K2HPO4 1 g
  Cellobiose 10 g
  Cysteine HCl 0.5 g
  Resazurin 1 mg
  Distilled water 1 L
  pH 6  
    Mix ingredients except cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add cysteine-HCl solution using sterile syringes.
    *Wako Pure Chemical Ind., Ltd., Osaka, Japan.