Search Results Detail

Medium Detail Information

Medium No.
1323
Medium
GV-medium
Composition
 K2HPO40.35g
 KH2PO40.23g
 NH4Cl0.5g
 NaCl2.25g
 FeSO4·7H2O2mg
 Trace element solution*1ml
 Selenite/tungstate solution**1ml
 Yeast extract1g
 Resazurin0.5mg
 NaHCO33g
 MgSO4·7H2O0.5g
 CaCl2·2H2O0.25g
 D-Glucose5g
 Vitamin solution***10ml
 L-Cysteine-HCl·H2O0.3g
 Na2S·9H2O0.3g
 Distilled water1L
  Dissolve ingredients (except bicarbonate, magnesium sulfate, calcium chloride, glucose, vitamins, cysteine and sulfide), bring medium to the boil, and then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate, adjust pH to 7.0, dispense under same gas atmosphere in culture vessels and autoclave. After sterilization, add magnesium sulfate, calcium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of the completed medium to 7.0 - 7.2, if necessary.
       
  *Trace element solution.
   HCl (25% or 7.7 M)10ml
   FeCl2·4H2O1.5g
   ZnCl270mg
   MnCl2·4H2O100mg
   H3BO36mg
   CoCl2·6H2O190mg
   CuCl2·2H2O2mg
   NiCl2·6H2O24mg
   Na2MoO4·2H2O36mg
   Distilled water990ml
  First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000 ml.
       
  **Selenite-tungstate solution.
   NaOH0.5g
   Na2SeO3·5H2O3mg
   Na2WO4·2H2O4mg
   Distilled water1L
       
  ***Vitamin solution.
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl·2H2O5mg
   Riboflavin5mg
   Nicotinic acid5mg
   D-Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
Comment