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Medium Detail Information

Medium No.
 Trace element solution*1ml
 Selenite/tungstate solution**1ml
 Yeast extract1g
 Vitamin solution***10ml
 Distilled water1L
  Dissolve ingredients (except bicarbonate, magnesium sulfate, calcium chloride, glucose, vitamins, cysteine and sulfide), bring medium to the boil, and then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate, adjust pH to 7.0, dispense under same gas atmosphere in culture vessels and autoclave. After sterilization, add magnesium sulfate, calcium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of the completed medium to 7.0 - 7.2, if necessary.
  *Trace element solution.
   HCl (25% or 7.7 M)10ml
   Distilled water990ml
  First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000 ml.
  **Selenite-tungstate solution.
   Distilled water1L
  ***Vitamin solution.
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L