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Medium Detail Information

Medium No.
Sulfur-Free Mineral Medium (SFMM)
  Disodium succinate 10 g
  Na2HPO4 2.2 g
  KH2PO4 0.8 g
  NH4NO3 3 g
  CaCl2·2H2O 10 mg
  MgCl2·6H2O 10 mg
  FeCl2·2H2O 10 mg
  Modified Hunter's vitamin-free mineral base* 20 ml
  Dimethyl sulfoxide** 5 ml
  Agarose (Electrophoresis grade, if needed) 5 g
  Distilled water 975 ml
  pH 7.2  
    *Modified Hunter's vitamin-free mineral base
      Nitrilotriacetic acid (NTA) 10 g
      MgCl2·6H2O 16 g
      CaCl2·2H2O 3.3 g
      (NH4)6MoO7O24·4H2O 9.25 mg
      FeCl3·6H2O 0.102 g
      Mofified metals 44*** 50 ml
      Distilled water 950 ml
    Dissolve and neutralized nitrilotriacetic acid in 500 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8, and bring volume to 1 L with distilled water.
    **Dimethyl sulfoxide
    Sterilize separately by filtration.
    ***Mofified metals 44
      Na-EDTA 2.5 g
      ZnCl2 5.17 g
      FeCl2·6H2O 4.85 g
      MnCl2·4H2O 2.02 g
      CuCl2·2H2O 0.267 g
      Co(NO3)2·6H2O 0.248 g
      Na2B4O7·10H2O 0.177 g
      Distilled water 1 L
    Dissolve the EDTA and add a few drops of 12M HCl to retard precipitation of the heavy metal ions. Store in the dark and cold (5℃).