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Medium Detail Information

Medium No.	1333
Medium	Basal Salt Medium with biphenyl
Composition
Distilled water 936 ml   20 x PO4* 50 ml   100 x (NH4)2SO4** 10 ml   1000 x FeSO4*** 1 ml   1000 x MgCl2**** 1 ml   1000 x Trace elements***** 1 ml   1000 x Na2MoO4****** 1 ml   Agar (if needed) 15 g         Sterilize separately and cool to 65°C. Aseptically add to the following medium in order.                   * 20 x PO4       K2HPO4 87.09 g       KH2PO4 68.05 g       Distilled water 1 L   pH 7.0       Adjust pH to 7.0 with KOH.                   ** 100 x (NH4)2SO4       (NH4)2SO4 198.66 g       Distilled water 1 L   pH 7.0       Adjust pH to 7.0 with NaOH.                   *** 1000 x FeSO4       FeSO4 0.056 g       Distilled water 100 ml                   **** 1000 x MgCl2       MgCl2 16.26 g       Distilled water 100 ml                   ***** 1000 x Trace elements       CaCl2 2.65 g       MnCl2·4H2O 0.1 g       Distilled water 100 ml                   ****** 1000 x Na2MoO4       Na2MoO4·4H2O 0.1 g       Distilled water 100 ml     Substrate (biphenyl) was added at a concentration of 1g/liter. For agar plating medium, biphenyl was provided as a vapor by placing crystals on the lid of a petri dish. The dish was sealed with polyethylene tape.
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