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Medium Detail Information

Medium No.
1351
Medium
Medium for sulfur-disproportionating bacterium
Composition
 MgCl2·6H2O0.2g
 CaCl2·2H2O0.1g
 NH4Cl0.1g
 KH2PO40.1g
 KCl0.1g
 Na2S2O3·5H2O5g
 NaHCO32.5g
 Trace element solution*2ml
 Vitamin solution**10ml
 Fe(III) slurry solution***50ml
 Distilled water1L
  Mix ingredients except for vitamin and Fe(III) slurry solutions, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave Fe(III) slurry solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, add vitamin and Fe(III) slurry solutions. Final pH is 6.5-7.0.
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  ***Fe(III) slurry solution
  Neutralize 0.4 M FeCl3·6H2O solution with NaOH, wash with pure water several times, and autoclave under a N2 atmosphere.
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