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Medium Detail Information

Medium No.	1351
Medium	Medium for sulfur-disproportionating bacterium
Composition
MgCl2·6H2O 0.2 g   CaCl2·2H2O 0.1 g   NH4Cl 0.1 g   KH2PO4 0.1 g   KCl 0.1 g   Na2S2O3·5H2O 5 g   NaHCO3 2.5 g   Trace element solution* 2 ml   Vitamin solution** 10 ml   Fe(III) slurry solution*** 50 ml   Distilled water 1 L     Mix ingredients except for vitamin and Fe(III) slurry solutions, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave Fe(III) slurry solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, add vitamin and Fe(III) slurry solutions. Final pH is 6.5-7.0.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       p-Aminobenzoic acid 1 mg       Vitamin B12 0.01 mg       Distilled water 1 L                   ***Fe(III) slurry solution     Neutralize 0.4 M FeCl3·6H2O solution with NaOH, wash with pure water several times, and autoclave under a N2 atmosphere.
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