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Medium Detail Information

Medium No.
Medium for sulfur-disproportionating bacterium
 Trace element solution*2ml
 Vitamin solution**10ml
 Fe(III) slurry solution***50ml
 Distilled water1L
  Mix ingredients except for vitamin and Fe(III) slurry solutions, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave Fe(III) slurry solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, add vitamin and Fe(III) slurry solutions. Final pH is 6.5-7.0.
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  ***Fe(III) slurry solution
  Neutralize 0.4 M FeCl3·6H2O solution with NaOH, wash with pure water several times, and autoclave under a N2 atmosphere.