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Medium Detail Information

Medium No.
Medium for Strain M6.X2
  Casein peptone, tryptic digest 10 g
  Yeast extract 5 g
  Meat extract 5 g
  Bacto Soytone 5 g
  Glucose 10 g
  K2HPO4 2 g
  MgSO4·7H2O 0.2 g
  MnSO4·H2O 0.05 g
  Tween 80 1 ml
  NaCl 5 g
  Inulin 1 g
  Cysteine-HCl·H2O 0.5 g
  Salt solution* 40 ml
  Vitamin solution** 10 ml
  Resazurin 1 mg
  Distilled water 950 ml
  pH 6.8  
    Mix ingredients except vitamin solution and cysteine-HCl. Flush with N2/CO2, and adjust pH to 6.8 using 8 N NaOH. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. Separately autoclave cysteine-HCl under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically and anaerobically add vitamin and cysteine-HCl.
    *Salt solution
      CaCl2·2H2O 0.25 g
      MgSO4·7H2O 0.5 g
      K2HPO4 1 g
      KH2PO4 1 g
      NaHCO3 10 g
      NaCl 2 g
      Distilled water 1 L
    **Vitamin solution
      Biotin 2 mg
      Folic acid 2 mg
      Pyridoxine-HCl 10 mg
      Thiamine-HCl·2H2O 5 mg
      Riboflavin 5 mg
      Nicotinic acid 5 mg
      D-Ca-pantothenate 5 mg
      Vitamin B12 0.1 mg
      p-Aminobenzoic acid 5 mg
      Lipoic acid 5 mg
      Distilled water 1 L