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Medium Detail Information

Medium No.	1407
Medium	PPLO broth for Mycoplasma arginini
Composition
Bacto PPLO Broth w/o CV (Difco) 14.7 g   Horse serum* 200 ml   25% Yeast extract solution** 100 ml   Phenol red 20 mg   L-Arginine 2.1 g   L-Glutamine*** 1.46 g   100X MEM Vitamins (Gibco) 1 ml   Agar (if needed) 15 g   Distilled water 600 ml   pH 7.1                     *Sterilize by filtration, and add to another component sterilized by autoclaving.                   **Boil 250 g of dried baker's yeast suspended in 1 L of distilled water for 30 min, remove yeast cells by centrifugation, and sterilize by filtration.                   ***Prepare 100 mM solution and sterilize by filtration.     The anaerobic growth on plates was carried out in an anaerobic jar equipped with a CO2-generating gas pack (Mitsubishi Gas Chemical Company Inc., Tokyo, Japan).
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