カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	1451
Medium	GYP medium (pH 6) for anaerobe
Composition
Glucose 10 g   Bacto Yeast Extract (Difco) 10 g   Hipolypepton* 5 g   Sodium acetate 2 g   Tween 80 solution** 10 ml   Salts solution*** 5 ml   Resazurin 1 mg   Cysteine-HCl·H2O 3 g   Distilled water 1 L   pH 6.0       Mix ingredients except cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2 gas and seal with butyl rubber stoppers. Separately autoclave cysteine-HCl as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine-HCl solution.                   *Wako Pure Chemical Ind., Ltd., Osaka, Japan.                   **Tween 80 solution       Tween 80 50 g       Distilled water 1 L                   ***Salts solution       MgSO4·7H2O 40 g       MnSO4·4H2O 2 g       FeSO4·7H2O 2 g       NaCl 2 g       Distilled water 1 L     Add a drop of 12 N HCl to 500 ml of the salts solution.
Comment