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Medium Detail Information

Medium No.
GYP medium (pH 6) for anaerobe 
 Bacto Yeast Extract (Difco)10g
 Sodium acetate2g
 Tween 80 solution**10ml
 Salts solution***5ml
 Distilled water1L
 pH 6.0 
  Mix ingredients except cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2 gas and seal with butyl rubber stoppers. Separately autoclave cysteine-HCl as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine-HCl solution.
  *Wako Pure Chemical Ind., Ltd., Osaka, Japan.
  **Tween 80 solution
   Tween 8050g
   Distilled water1L
  ***Salts solution
   Distilled water1L
  Add a drop of 12 N HCl to 500 ml of the salts solution.

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