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Medium Detail Information

Medium No.
1504
Medium
Modified Biebl and Penning's Medium
Composition

 

KH2PO4 0.5 g
MgSO4·7H2O 0.2 g
NaCl 0.4 g
NH4Cl 0.6 g
CaCl2·2H2O 0.05 g
Sodium pyruvate 3 g
Yeast extract 1 g
Peptone 1 g
Ferric citrate solution* (0.1%) 5 ml
Vitamin solution** 2 ml
Trace element solution SL7*** 1 ml
Distilled water 1 L
  pH 7  

Mix ingredients except for vitamin solution and autoclave under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add vitamin solution sterilized by filtration.

*Soluble in hot water.

**Vitamin solution

Biotin 2 mg
Folic acid 2 mg
Pyridoxine-HCl 10 mg
Thiamine-HCl 5 mg
Riboflavin 5 mg
Nicotinic acid 5 mg
Ca-pantothenate 5 mg
p-Aminobenzoic acid 1 mg
Vitamin B12 0.01 mg
Distilled water 1 L
 

***Trace element solution SL7

HCl (25%; 7.7 M) 10 ml
FeCl2·4H2O 1.5 g
ZnCl2 70 mg
MnCl2·4H2O 100 mg
H3BO3 60 mg
CoCl2·6H2O 200 mg
CuCl2·2H2O 20 mg
NiCl2·6H2O 20 mg
Na2MoO4·2H2O 40 mg
Distilled water 990 ml
 

Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.

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