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Medium Detail Information

Medium No.
R2A + fumarate (anaerobic)
Bacto Yeast Extract (Difco) 0.5 g
Bacto Proteose Peptone No. 3 (Difco) 0.5 g
Bacto Casamino Acids (Difco) 0.5 g
Glucose 0.5 g
Soluble starch 0.5 g
Sodium pyruvate 0.3 g
Sodium fumarate 1.4 g
K2HPO4 0.3 g
MgSO4·7H2O 0.05 g
Agar (if needed) 15 g
Distilled water 1 L
  pH 6-7  

For anaerobic liquid medium, dispense the medium into suitable culture vessels under a N2/CO2 atmosphere, seal with butyl rubber stoppers, and autoclave at 121°C for 20 min.